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Australian Centre for Ancient DNA
School of Earth & Environmental Sciences
Darling Building
THE UNIVERSITY OF ADELAIDE
SA 5005
AUSTRALIA
Email

Telephone: +61 8 8313 3952
Facsimile: +61 8 8313 4364

ACAD Bioinformatics Early Career Researcher Workshop, 5th - 9th November, 2012
The University of Adelaide, North Terrace Campus

Sponsored by the Environment Institute

ACAD is pleased to host its 3rd ECR workshop that builds on the very successful workshops in Mathematical Biology (2009) and Phylogenetics (2010).  This workshop is an intensive 5 day hands-on training course tutored by international experts in the field, utilising leading software packages for the analysis of Next Generation Sequencing (NGS) and SNP array data.  The tutors will provide detailed instructions on the analysis of model and empirical datasets, and focus on the latest techniques available.  Early registration is strongly encouraged as seating is limited to 30 places, and there is strong demand.  Interested graduates are encouraged to apply for travel funds from their host institution.

Pre-requisites: Delegates must be familiar with UNIX environments and basic command lines, and will require a basic knowledge of the technology and analytical tools of NGS. 

Aims of the workshop: Delegates will gain a broad understanding of the latest bioinformatics tools and methods to handle and analyse NGS and SNP array data.  The course will focus on interpreting complex data sets, such as genomic and metagenomic samples, and review the most recent developments in the field.  Methods to deal with damaged and low copy number templates will be included.   

Organising Committee

Members of the Australian Centre for Ancient DNA, School of Earth & Environmental Sciences:
Professor Alan Cooper
Dr Bastien Llamas
Dr Julien Soubrier
Ms Maria Lekis.

Acknowledgement goes to Dr Stephen Bent and Dr Nathan Watson-Haigh for their invaluable IT assistance.

Presenters

Stephen Bent

Stephen Bent

Stephen received his PhD in Bioinformatics and Computational Biology from the University of Idaho, working on problems in microbial ecology. Research on pathogen population genetics in tick-borne disease followed at the Yale School of Public Health, and since arriving in Adelaide, he has been working with prokaryotic and eukaryotic second-generation sequencing data sets and performing transcriptome, genome, and metagenome analyses. Stephen's principal intersts lie in facilitating "aha!" moments and understanding microbial population patterns and processes.

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Ludovic Orlando

Ludovic is leading a research group at the Centre for GeoGenetics, Natural History Museum of Denmark, Copenhagen, where his group is developing integrative approaches in ancient DNA research, promoting the field of palaeomics by merging together biochemsitry, molecular biology, genomics and computational biology. These novel approaches have recently specifically been used to study the evolutionary history of one iconic mammal: the horse.

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Martin Kircher

Martin's training was in Computational Molecular Biology and entered the field of ancient DNA at a time where high-throughput sequencing completely transformed Svante Pääbo's department at the Max Planck Institute for Evolutionary Anthropology. He developed a base caller for the Illumina/Solexa systems, implemented the analysis pipelines that underlie the Neandertal and Denisova genomes and analyzed NGS data for comparative gene expression studies. Currently, Martin is currently at the University of Washington with Jay Shendure's group.

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Laura Wegener Parfrey

Laura did her PhD at the University of Massachusetts and focused on resolving the eukaryotic tree of life by incorporating diverse microbial lineages into phylogenetic analyses. Laura is currently a Postdoc at the University of Colorado where is is using her experitse in eukaryotes to incorporate them into Qimme, where she has used this software extensively for analyses of microbial communities using 16S and 18S marker genes.

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Bob Schnabel

A geneticist by training, Bob Schnabel has increasingly moved into a bioinformatics role to support the large amount of data being generated by SNP chips and NGS. Bob was a member of the consortia that developed the Illumina BovineSNP50, BovineHD and Affymetrix BOS-1 genotyping arrays. He was also a leader for the QA/QC group for the Bovine HapMap Consortium."

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Program

  Mon 5th Nov Tue 6th Nov Wed 7th Nov Thu 8th Nov Fri 9th Nov
Includes morning tea break, lecture and hands on sessons

Welcome, Introductions and set up of PC logons.

Stephen Bent: Referesher on command lines and UNIX Environment (grep, awk, other shell one-liners and pipelines).  Includes outlines on public databases (UCSC, ENSEMBL) 

Martin Kircher:

Introduction on NGS platforms, data acquisition and analyses

Ludovic Orlando:

Brief introduction on ancient DNA, with the problems of contamination, low endogenous contents and post-mortem damage. Use of 2nd + 3rd generation sequencing for the anlysis of ancient DNA.

Laura Wegener Parfrey:

Metagenomics: Assessing microbial community patterns using Qiime (quantitative insights into microbial ecology).

Bob Schnabel:

Introduction  to SNP quality analysis: Managing data (SNP and NGS) and quality control issues prior to analysis.

Lunch break 
Hands on sessions, includes afternoon tea break

Stephen Bent:

Practical application of the morning's topics. 

Martin Kircher:

NGS pipelines and tools.

Ludovic Orlando: Mapping ancient DNA Illumina reads to reference genomes to highlight damage patterns and SNP variation. Published data on the Black Death will be used to illustrate this session.

Laura Wegener Parfrey:

Particpants will go through tutorial data using Qiime to process raw data and then assess diversity patterns in microbial communities.

Debrief on the essential sessions of the workshop. 

PUBLIC LECTURES
5-6pm

Mawson L.T.

Martin Kircher: A high coverage Denisovan genome - what 30x coverage from a more than 50,000 old pinky bone tells us about her and our past. Ludovic Orlando: The last million years of horse evolution. Laura Wegener-Parfrey:
The eukaryotic tree of life as a framework for eukaryotic community analyses.

Bob Schnabel: Application of whole genome sequencing to identify Mendelian disease candidates in dogs using a 1-case + N-control design.

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 Registration

 

Registration now closed. Seats have sold out.

 

Registration fees:

  • Early Bird registration AUD $400.00 (includes training manual and welcome breakfast). Deadline date 3rd September, 2012
  • Non Early Bird registration AUD $450.00 (includes training manual and welcome breakfast).
  • For Environment Institute members, there is a special Early Bird offer, please contact Raeleen Day to apply for the reduced rate.
  • NO SINGLE DAY REGISTRATION AVAILABLE

Payment registration form download here. Regisration form plus full payment must reach our office no later than 30 September, 2012. Late payment does not guarantee a placing.

Completed registration payment form can be returned via scanned pdf only and emailed as an attachment to: 
Maria Lekis
c/- Australian Centre for Ancient DNA or delivered by hand at the Centre office, Room 209a Darling Building
North Terrace Campus
The Universsity of Adelaide

For enquiries phone +61 8 8313 3952 | Email Maria here

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Venue details and Map

North Tce Campus

This 5 day workshop is held at the University of Adelaide, North Terrace Campus
Lectures/presentations are held in the Mawson Lecture Theatre, Ground Floor, Mawson Building
Hands on sessions are held in the Mawson Computer Suite, Ground floor, Rm G12, Mawson Building

Click campus map left for enlarged image. Mawson Building map reference 11B

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Adelaide Accommodation

The following is a list of accommodation venues we normaly use for their  close proximity to the University and affordability:

1) Mantra on Frome. approx 10mins walk to the University. Contact phone +61 8 8412 3333. Standard rooms range from AUD$110-130

2) Adelaide Riviera Hotel, North Terrace, City. Standard room is around AUD $125.00 and approx 10mins walk to the University. Contact phone +61 8231 8000 or Toll free in Australia only # 1800 061 300

3) The Vines or Grand Apartments in North Adelaide City. Standard rooms range from AUD $79-$100. approx 10mins walk to the University. Contact phone Toll free in Australia only #1300 668 400

4) The Oaks Embassy 96 North Terrace, Adelaide. 1 bedroom apartment from AUD $134.00.

4) Try the site Backpacker and Youth/Student Hostels in Adelaide where a listing of local backpackers is provided.

5) Another site for students who are willing to share accommodation - check EasyRoommate or Adelaide Backpackers Accommodation

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