Associate Professor Mark Nottle

Research Interests

The Reproductive Biotechnology group has an international reputation in the general areas of reproductive biology and the development of associated technologies for biomedical and agricultural applications. In collaboration with a number of university, institute and hospital research groups in Australia as well as overseas, current research is focused on developing organ, tissue and cell replacement therapies. This work is funded by various agencies including the Juvenile Diabetes Research Foundation, the National Health and Medical Research Council and industry. In 2009 the group presented its findings regarding the development of a new method for the isolation of mammalian embryonic stem cells including its use in isolating embryonic stem cells from the pig.

Research Priority:
Isolation and characterisation of various stem cell types from the pig. These will be used for a range of biomedical and agricultural applications, as well as a model for human stem cell research.

Publications

Sow litter size is increased in the subsequent parity when lactating sows are fed diets containing omega 3 fatty acids from fish oil.

Smits RJ, Luxford BG, Mitchell M, Nottle MB.
J Anim Sci. 2011 May 24.

 

Isolation and in vitro characterization of putative porcine embryonic stem cells from cloned embryos treated with trichostatin A.

Vassiliev I, Vassilieva S, Truong KP, Beebe LF, McIlfatrick SM, Harrison SJ, Nottle MB.

Cell Reprogram. 2011 Jun;13(3):205-13. 

Versatile co-expression of graft-protective proteins using 2A-linked cassettes.
Fisicaro N, Londrigan SL, Brady JL, Salvaris E, Nottle MB, O'Connell PJ, Robson SC, d'Apice AJ, Lew AM, Cowan PJ.
Xenotransplantation. 2011 Mar-Apr;18(2):121-30.

Piglets produced from in vivo blastocysts vitrified using the Cryologic Vitrification Method (solid surface vitrification) and a sealed storage container.
Beebe LF, Bouwman EG, McIlfatrick SM, Nottle MB.
Theriogenology. 2011 May;75(8):1453-8.

On the need for porcine embryonic stem cells to produce Gal KO pigs expressing multiple transgenes to advance xenotransplantation research.
Nottle MB, Vassiliev I, O'Connel PJ, d'Apice AJ, Cowan PJ.
Xenotransplantation. 2010 Nov-Dec;17(6):411-2.

Genetic modification of pigs for solid organ xenotransplantation.
Gock H, Nottle M, Lew AM, d'Apice AJ, Cowan P.
Transplant Rev. 2011 Jan;25(1):9-20.

Development of a Mouse Model for Studying the Effect of Embryo Culture on Embryonic Stem Cell Derivation.
Campbell JM, Mitchell M, Nottle MB, Lane M.
Stem Cells Dev. 2011 Mar 17.

In vitro and in vivo characterization of putative porcine embryonic stem cells.
Vassiliev I, Vassilieva S, Beebe LF, Harrison SJ, McIlfatrick SM, Nottle MB.
Cell Reprogram. 2010 Apr;12(2):223-30.

Reproductive responses to daily injections with porcine somatotropin before mating in gilts.
Gatford KL, Grupen CG, Campbell RG, Luxford BJ, Smits RJ, Owens PC, Nottle MB.
J Reprod Dev. 2010 Oct;56(5):540-5.

Development of culture conditions for the isolation of pluripotent porcine embryonal outgrowths from in vitro produced and in vivo derived embryos.
Vassiliev I, Vassilieva S, Beebe LF, McIlfatrick SM, Harrison SJ, Nottle MB.
J Reprod Dev. 2010 Oct;56(5):546-51.

Maternal responses to daily maternal porcine somatotropin injections during early-mid pregnancy or early-late pregnancy in sows and gilts.
Gatford KL, Smits RJ, Collins CL, Argent C, De Blasio MJ, Roberts CT, Nottle MB, Kind KL, Owens JA.
J Anim Sci. 2010 Apr;88(4):1365-78.

Cytochalasin B and trichostatin a treatment postactivation improves in vitro development of porcine somatic cell nuclear transfer embryos.
Beebe LF, McIlfatrick SJ, Nottle MB.
Cloning Stem Cells. 2009 Dec;11(4):477-82.

Responses to maternal GH or ractopamine during early-mid pregnancy are similar in primiparous and multiparous pregnant pigs.

Gatford KL, De Blasio MJ, Roberts CT, Nottle MB, Kind KL, van Wettere WH, Smits RJ, Owens JA.

J Endocrinol. 2009 Oct;203(1):143-54.

 

Effect of follicle size and dibutyryl cAMP on the cAMP content and gap junctional communication of porcine prepubertal cumulus-oocyte complexes during IVM.

Bagg MA, Nottle MB, Armstrong DT, Grupen CG.

Reprod Fertil Dev. 2009;21(6):796-804

 

Adding essential amino acids at a low concentration improves the development of in vitro fertilized porcine embryos.

Beebe LF, Vassiliev I, McIlfatrick S, Nottle MB.

J Reprod Dev. 2009 Aug;55(4):373-7.

 

A comparison of two in vitro maturation media for use with adult porcine oocytes for adult somatic cell nuclear transfer.

Beebe L, McIlfatrick S, Grupen C, Boquest A, Harrison S, Faast R, Ashman R, Wengle J, Hamilton H, Nottle M.

Cloning Stem Cells. 2007 Winter;9(4):564-70.

 

Relationship between follicle size and oocyte developmental competence in prepubertal and adult pigs.

Bagg MA, Nottle MB, Armstrong DT, Grupen CG.

Reprod Fertil Dev. 2007;19(7):797-803.

 

Production of homozygous alpha-1,3-galactosyltransferase knockout pigs by breeding and somatic cell nuclear transfer.

Nottle MB, Beebe LF, Harrison SJ, McIlfatrick SM, Ashman RJ, O'Connell PJ, Salvaris EJ, Fisicaro N, Pommey S, Cowan PJ, d'Apice AJ.

Xenotransplantation. 2007 Jul;14(4):339-44.

 

The effect of energy substrate concentration and amino acids on the in vitro development of preimplantation porcine embryos.
Beebe LF, McIlfactrick S, Nottle MB.
Cloning Stem Cells. 2007 Summer; 9(2):206-15.

 

Use of adult mesenchymal stem cells isolated from bone marrow and blood for somatic cell nuclear transfer in pigs.

Faast R, Harrison SJ, Beebe LF, McIlfatrick SM, Ashman RJ, Nottle MB.

Cloning Stem Cells. 2006 Fall;8(3):166-73.

 

Effect of dibutyryl cAMP on the cAMP content, meiotic progression, and developmental potential of in vitro matured pre-pubertal and adult pig oocytes.

Bagg MA, Nottle MB, Grupen CG, Armstrong DT.

Mol Reprod Dev. 2006 Oct;73(10):1326-32.

 

Entry last updated: Thursday, 11 Aug 2011