Analysis Tools

• Biacore
• Confocal Microscopy
• FACS Analysis
• Mass Spectrometer
• Molecular Imager

Biacore

The School's Research Support team provides real-time biomolecular interaction analysis (BIA) using the Biacore 2000. BIA can be used to study the interactions of proteins, protein conjugates, nucleic acids, lipid micelles as well as larger particles such as viruses and whole cells.

Cost:

• $120 per day (charged in half day blocks) + consumables, which are charged to clients at cost price.

For more information contact Chris Cursaro or John Mackrill on +61 8 8303 5262.

Confocal Microscopy

This facility has a confocal microscope which uses a krypton/argon laser and COMOS software. It is suitable for both single and double labelled fluorescent samples. The Olympus microscope is of the inverted type, and is equipped with phase contrast condensor, transmission detector, and a variety of apochromatic and achromatic objectives. Confocal Assistant software is available for handling of image files.

Cost:

• Without Technical Operator Assistance $40.00 per hour
• With Technical Operator Assistance $80.00 per hour
• Training $250 for 2 x 2 hour sessions

Policy:

• Minimum charge is 1 hour
• Maximum booking time is a 2 hour block (except by special arrangement)

Please contact Dr. Renato Morona 08 8303 4151 or Mr Chris Cusaro 08 8303 7521 for further details.

FACS Analysis

The School has fluorescence-activated cell scanning capabilities. It is planning to upgrade our capacity in this area to include high-speed sorting and high throughput analysis. Expert assistance will be available and a small charge will be applied to cover costs and maintenance.

For more information and costs of this service, please contact Chris Cursaro or John Mackrill on +61 8 8303 5262.

Mass Spectrometer

Mass Spectrum Analysis of purified proteins and peptides is available with the Micromass Q-TOF hybrid quadrupole time of flight mass spectrometer. Extremely accurate determinations of molecular mass can be made with this instrument. It is also capable of assisting with structural investigations, using the hexapole collision cell, between the two mass analysers.

Cost:

• Molecular weight determination $60.00 per sample

For further information, please contact Chris Cursaro on 8303 7521.

Molecular Imager

This facility is based on a BioRad FX Imager. This versatile instrument uses laser based technology and Quantity One software to scan, capture and quantify both radio-isotope and fluorophor labelled molecules. Imaging plates, gels, trays and plates, in a variety of formats, can be scanned. A variety of wavelengths are available to detect commonly used fluorescent molecules.

Please contact Mr Chris Cusaro for further details.