Fusion and subcloning
We can use NS-1 and/or SP2/0 myeloma cell lines for the fusion to splenocytes from immunised Balb/c mice.
Fusion is performed according to standard PEG fusion protocols optimised in our laboratory and the cells plated in 48-well and 96-well plates to grow and isolate viable hybridoma cell.
After initial testing, all positive clones will be selected and expanded. Culture supernatant from the positive cultures will be sent to the customer's laboratory for further testing and confirmation.
Clones selected by the customer will be subcloned until cell line is deemed to be monoclonal and expanded for cryopreservation.
Any unwanted clones will be destroyed after consultation with the client.