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Further Enquiries:
School of Molecular & Biomedical Science
The University of Adelaide
SA 5005
Australia
Email

Reception
Molecular Life Sciences building:

Telephone:  +61 8 8313 5352
Telephone: +61 8 8313 5328

Facsimile: +61 8 8313 4362

Using PC2 Laboratory MLS G.50

Training & Registration
All users of G.50 will be registered with the University IBC as authorised to work in PC2 areas, recorded in the MBS training records as having read the School PC2 Manual, Handling GMO and Pathogenic Virus Systems http://www.adelaide.edu.au/mbs/safety/biosafety/ GMOPathVirus.html, and this document.

General Lab procedures
Work practices will follow the School PC2 manual unless directed otherwise by this document and/or Handling GMO and Pathogenic Virus Systems.

Disinfection:
In this laboratory the disinfectant of choice, to inactivate viruses, is 1.25% Chlorine solution.  This is made by diluting Sodium Hypochlorite solution by the appropriate amount.  Biogram is used to control bacterial growth in the aspiration pump traps.  Ethanol solutions may be used to dry surfaces and rinse away chlorine solutions.

  • The aspiration pumps are not used to aspirate any viral infected liquid.
  • Traps of pumps are be emptied when full and then Biogram will be put in the trap before using (fill to 1/60th of the trap volume).
  • No glass sharps or needles to be used when working with virus.
  • Tips will be rinsed up and down with 1.25% Chlorine solution before ejecting into waste containers. These are then capped or placed in another bag before disposing in the main autoclave bags and then into the yellow bins.
  • Stocks of 12.5% available chlorine sodium hypochlorite solution, 100% ethanol and Biogram concentrate will be held in G.50.
  • Incubators are to be monitored by everyone for cleanliness and  water in the bottom tray. Incubators are to be cleaned on a regular basis by users.
  • If using unsealed containers for cells i.e. Petri dishes or multi-well plates: these will be placed on a tray in the incubator to control spillage.
  • At the completion of work all surfaces will be swabbed with 1.25% Chlorine solution followed by an ethanol swab to rinse away the chlorine.
  • UV irradiation is not effective for inactivating virus.
  • Viral culture waste will be disposed of into a flask, containing Chlorine solution, within the Biosafety hood. This solution must be prepared daily by placing one tenth the flasks total volume of 12.5% available chlorine sodium hypochlorite solution into the flask.
  • Separate waste flasks will be used for adenoviral/lentiviral and HCV waste.
  • Waste will be held in the flasks for a minimum of 15 minutes before disposal to sewer.

Waste Disposal

  • All infected waste will be double contained
  • Yellow Bins: locked full bins will be exchanged for empty bins when ½ to ¾ full.  Full and replacement empty bins are held in the Ground floor storage compound.
  • Liquid waste flasks the containers are to be swabbed, with fresh 1.25% chlorine solution, and taken into the Liquid N2 room to be poured down the sink.
  • The content of liquid waste containers will not be disposed of in the plastic bags/Yellow bins.

Tony Richardson
21/01/2010