Fish in the Facility
The Adelaide Zebrafish Facility aquarium is comprised of two separated
colonies of various wild-type and transgenic fish with a maximum capacity
of approximately 17,800 fish.
Wild Type Strains:
AB
Abbreviation: AB
ZFIN
ID: ZDB-GENO-960809-7
The AB line is the primary background of all transgenic and mutant fish that
come from the Zebrafish International Resource Stock Center (ZIRC), in Oregon,
USA.
The AB line was originally derived from two lines, A and B, purchased
by Streisinger at
different times from a pet shop in Albany, Oregon. The original A and
B lines probably originated from a hatchery in Florida.
Haploid offspring from individual females of the AB line were screened for
healthy, good looking embryos, and those females were used to make future
generations by crossing them to unselected males. The AB line was maintained
in this manner by the Oregon labs from the 1970's to the 1990's. This
procedure reduced the number of lethal mutations so that this line could
be used for parthenogenesis.
By 1991 the AB fish were 70 generations removed from the original wild-type
fish that George Streisinger and Charline Walker used to establish the AB
line.
This stock is currently called AB, even though it is only distantly related
to the original Streisinger AB line.
Hybrid
Abbreviation: Hyb
The hybrid stock was originally set up to maintain good out breeding practices
and produce a level of hybrid vigour in the Facility's wild type strains. The
Hyb stock was first set up as a cross between adults from the WT and I1 populations.
Today's hybrid stock is developed from various couplings of the wild-type
strains, and is regenerated after 5 generations.
Introduced Strain 1
Abbreviation: I1
This line of wild type fish was an introduction of a second pet shop purchase
in 2002.
Leopard
Abbreviation: Leo
The leopard stock was generated from a naturally occurring mutation in the
WT lines. The Leo phenotype is a result of a recessive mutation (leot1)
causing spotting in adult fish, also known as tup. This strain
is very useful for managing animal screens, as the spots clearly differentiate
the animal of interest from its screening partner.
Longfin Leopard
Abbreviation: LofLeo
Homozygous for leot1 and lofdt2,
this strain is a long finned variant of the Leo stock, and is also used in
screening. It was generated by mating Leo females with a long finned
mutant male that arose in the WT stock in 2004. The long finns are
caused by the mutation known as lofdt2, a dominant homozygous
viable mutation.
Tuebingen
Abbreviation: TU
ZFIN
ID: ZDB-GENO-990623-3
The TU line is the primary background for any fish originating from the Tuebingen
Zebrafish Stockcenter. The strain housed at the AZF was obtained
from ZIRC as a clutch of embryos.
The TU strain was selected by the Sanger Institute for the Zebrafish
sequencing project, and thus is the most relevant strain for many of our
users to work with. The reason the TU line was selected by Sanger was
because its genetic background had been cleaned up to remove embryonic lethal
mutations before being used for mutagenesis and sequencing in the Nüsslein-Volhard
Lab.
For more information on the Zebrafish genome project see the
Danio rerio sequencing project.
Wild-Type
Abbreviation: WT
The WT designation identifies fish that have come from our original pet shop
purchase in 1998.
WIK
Abbreviation: WIK
ZFIN
ID: ZDB-GENO-010531-2
The WIK line is very polymorphic relative to the TU and AB lines making it
an ideal candidate for use in genetic mapping screens for fish developed
on either the TU or AB background.
WIK was first described as WIK11 in "A
polymorphic Zebrafish line for genetic mapping using SSLPs on high-percentage
agarose gels" (1997) by Gerd-Jorg Rauch, Michael Granato, Pascal
Haffter.
Mutant & Transgenic Lines:
Fli1GFP(Vascular endothelium
and branchial arch expression)
Spt (Mesoderm development,
and specific neuron marker)
Spt:GFP (Spadetail
promoter linked to GFP)
Aβ(Human amyloid
beta 42 under the nacre promoter, melanocyte expression)
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