Quantitative Fluorescence
FISA
Fluorescence Imagers (South Australia) together with Adelaide Microscopy presented a symposium on Tuesday September 19, 2006 entitled
The program focused on various techniques in fluorescence microscopy (both wide-field and confocal) and how these may be used to obtain quantitative (or semi-quantitative) experimental data.
The potential of various fluorescence methods was presented, together with their limitations. Special consideration was given not only to the various specialised experimental techniques, but also to the overall collection of data and of its analysis. Speakers were able to present their own approach to tackling experimental problems with quantitating fluorescence data. Many participants were able to follow up with the various speakers to discuss their own applications during the breaks.
Speakers were Dr Meredith Wallwork (Adelaide Microscopy, University of Adelaide), Dr Stephen Gregory (University of Adelaide), Dr Peter Kolesik (Bionomics SA), Dr Lavinia Thaliana (Invitrogen), Dr Tatiana Shandala (Institute of Medical and Veterinary Science), Ms Kirsten Farrand (University of Adelaide), Mr Craig Noble (Olympus Australia), Professor Greg Barritt (Flinders University) and Dr Tak Kee (University of Adelaide).
Participants appreciated being able to approach the speakers who had generously given time to attend and were often able to obtain valuable assistance and advice.
| Speakers | |
|---|---|
 |
Professor Greg Barritt (Flinders University) |
 |
Dr Peter Kolesik (Bionomics SA) and Dr Meredith Wallwork (University of Adelaide) |
 |
Dr Tak Kee (University of Adelaide) |
 |
Dr Stephen Gregory (University of Adelaide) |
| The program for the day: | |
|---|---|
| Session 1 | Techniques and limitations to quantifying fluorescence (including co-localisation), FRET and FLIM. Industry application – high throughput fluorescence quantitation. |
| Session 2 | Immunolabelling techniques, choosing a fluorescence tag, multiple labelling and analysis of image data. |
| Session 3 | Ratio imaging (Calcium), other techniques including multiphoton microscopy, SHG (Second Harmonic Generation). |
Sponsorship provided by:
enabled participants to take part without cost.
In all, there were approximately 75 registrants on the day
from a wide range of research institutions in South Australia and feedback
indicated it was a useful and informative day.
Informal drinks and nibbles brought the day to a close.
Fluorescence Imagers (South Australia) and Adelaide Microscopy
Quantitative Fluorescence Symposium on quantitation techniques in fluorescence microscopy
Tuesday September 19
-Click on the below presentation links to get the printable version in PDF.Session 1 – The practical issues
9.00 am Dr Meredith Wallwork Welcome (University of Adelaide) Introduction
to Quantitative Fluorescence Practical Aspects of Image Collection
9.30 am Dr Stephen Gregory Re-defining colocalisation - Fluorescence Resonance Energy (University of Adelaide) Transfer (FRET) and Fluorescence Lifetime Imaging (FLIM)
10.00 am Dr Peter Kolesik Fluorescence Cell-based High Throughput Assays for Drug (Bionomics SA) Discovery
10.30 am MORNING TEA
Session 2 – Immunofluorescence
11.00 am Dr Lavinia Taliana Advances in Labelling and Detection (Invitrogen)
11.30 am Dr Meredith Wallwork Choices and Limitations to Quantitation of (University of Adelaide) Immunofluorescence
12.00 noon Dr Tetyana Shandala Zenon System for Fluorescence Antibody Labelling (IMVS / University of Adelaide)
12.30 pm Kirsten Farrand Quantitative Analysis of Multi-labelled Cells (University of Adelaide), Craig Noble Digital Imaging (Olympus Australia)
1.15 pm LUNCH Provided by
Session 3 – Specific applications, tricks and tips
2.15 pm Prof. Greg Barritt Calcium
Concentrations in Cytoplasmic Space and Organelles of (Flinders University)
Living Cells
2.45 pm Dr Tak Kee Biological Imaging using Multiphoton Spectroscopy (University of Adelaide)
3.15 pm Dr Meredith Wallwork Concluding
remarks (University of Adelaide) Some
Tricks and Tips! & quick
notes
3.30 pm Drinks and nibbles
Other notes
Quantitation of indirect immunofluorescence on paraffin sections with confocal microscopy
