Multi-Photon Microscope

Multiphoton SHG LaVision Biotec TriM Scope

Multiphoton fluorescence microscopy (MFM) also known as two-photon microscopy uses pulsed long wave-length (infra-red) laser light to excite fluorophores within the specimen being observed. The longer wavelength typically infra-red) excitation lasers have a low energy and are useful for visualisation of cell and tissue structure and function. Typically inorganic crystals and some biological samples, such as collagen, give rise to intense second harmonic light when irradiated with a laser allowing high quality imaging without altering the samples with fluorescence probes (required for standard fluorescence microscopy) therefore, the molecules shouldn't suffer the effects of phototoxicity or photobleaching.