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OLYMPUS BX51 - Research Grade Optical Microscope DIC, Phase,DF, BF
Thanks to Olympus
Australia, an Olympus BX51 upright microscope has been placed at Adelaide
Microscopy. This research microscope features 4X, 10X, 20X, 40X, 63X (oil)
and 100X (oil) lenses. The capabilities of the BX51 include Nomarski differential
interference contrast (DIC), fluorescence, dark-field and polarization
microscopies. Five long-pass filters are available for fluorescence microscopy.
These filters and examples of the fluorophores for which they can be used
are:
Ultra-violet (UV): DAPI
Blue-violet (BV): Lucifer yellow
Intermediate blue (IB): GFP, EGFP, FITC, Alexa 488
Intermediate green (IG): CY3, DS red, TRITC, RITC
CY5: CY5
The microscope is fitted with Soft Imaging System’s Colorview III
camera and AnalySIS Life Sciences software. The Colorview III camera can
record 24-bit colour images at resolutions up to 2576 X 1932 pixels and
16-bit monochrome images at resolutions up to 1288 X 966 pixels. The AnalySIS
software fully controls and configures the Colorview III camera and has
features that simplify the recording of images. These features include
a system for sequentially recording fluorescence images from different
fluorophores without the need to adjust exposure times thereby reducing
the time to record the images and minimising photo-bleaching. The AnalySIS
software can be used to perform complex image analysis on captured images
and is fully compatible with the other AnalySIS
systems at Adelaide Microscopy.
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