Generation of Conditional Alleles
An exciting development in the genome editing field was the recent demonstration that a conditionally targeted allele (containing LoxP sites flanking a critical exon) could be generated using CRISPR technology - Yang et al (2013) Cell 154, 1-10.
SAGE is currently assessing the efficacy of this published approach and other strategies for conditional allele generation. Given the widespread use of conditional alleles, SAGE can offer to generate conditional alleles on a collaborative basis for a significant discount.
Please contact SAGE for a quote.