Generation of point mutation and epitope tag alleles
Using the method of Yang et al (2013) Cell 154, 1-10, SAGE has generated point mutations and epitope-tagged (HA-FLAG) alleles (unpublished data).
All prices for SA Genome Editing projects includes: design and preparation of the genome editing agent(s) ie CRISPR gRNA, injection into mouse zygotes (3 sessions maximum), transfer of injected zygotes to pseudopregnant females, and screening and sequence verification of mutant alleles in founder mice.
A deposit of 50% of the total fee is required prior to commencement of the project and is non-refundable. The remaining fee is due upon delivery of the mutant mice. Mouse transport costs are not included in the SAGE fee. A discount is available for Robinson Research Institute and University of Adelaide members
Please contact SAGE for a more information and to discuss costs.