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Dr Michelle Lane
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Our Research Group studies the how the oocyte, sperm contribute to the development of the early embryo and how this programs the development of the pregnancy and affects offspring. Using state of the art techniques we are unravelling the biochemical and molecular basis for how a healthy embryo is made. In particular we ae focussing on epigenetic mechanisms including methylation and microRNAs. We also have an interest in embryonic stem cells.
We are at the fore-front of developing fundamental knowledge in oocyte development, spermatogenesis and embryogenesis to both develop new treatment therapies.
We also have a vast translational research program where we use this fundamental knowledge to improve IVF treatment for infertile couples. We work closely with world-leading IVF units Repromed and Monash IVF. We have several patents and have developed several new treatment regimes for IVF patients including the formation of sequential media and blastocyst development, vitirification and in metabolic assessment of embryos.
Her research group is currently active in understanding how lifestyle factors such as obesity and ageing impact on oocytes, sperm and embryos.
Michelle is an NHMRC Research Fellow, an inaugural Fellow of Reproductive Biology of the SRB, and in 2012 and 2013 her group has one several prizes at International meetings.
She received her PhD in 1996 and has published over 150 peer-reviewed papers, reviews and book chapters. She is internationally recognised for her work in understanding the biology of gametes and embryos and her team are frequently invited to give talks both nationally and internationally.
Our group is very active in student teaching.
We have several Honours and PhD students in our lab, and graduates from our lab are dotted around the world and Australia continuing to develop their science careers.
Current available PhD projects involve:
Gamete and Embryo Biology Laboratory
Basic Research informing Clinical Practice
Our research team is about studying fundamental biology questions that will directly impact on patient outcomes.
Our interests are in exploring the underlying cause of infertility and to develop better treatment strageties to improve the success of human assisted reproductive technologies. We collaborate closely with Repromed, South Australia's largest IVF unit.
Our research interests include:
Mammalian Oocyte Biology
Mammalian Preimplantation Development
Epigenetic control of early development
Impact of embryo health on pregnancy and offspring health
We also have a special interest in obesity and infertility.
Dr. Michelle Lane (Female and Male infertility and Nutrition/Obesity)
Dr. Tod Fullston (Molecular/Epigenetic regulation of gametes and embryos) (NHMRC Career Development Fellow)
Nicole Palmer (Obesity and offspring health/Andrology)
Wan Xian Kang
Nicole Palmer (PhD Student)
Sarah Wakefield (PhD student)
Leanne Pacella (PhD student)
Dr. Deirdre Zander-Fox (Environment and embryos/IVF technology)
Dr. Hassan Bakos (Male infertility)
Dr Kathryn Gebhardt (Viability markers for human embryos)
>150 peer reviewed journal articles; H-factor 47.
2. Fullston T, Ohlsson Teague EM, Palmer NO, Deblasio MJ, Mitchell M, Corbett M, Print CG, Owens JA, Lane M. Paternal obesity initiates metabolic disturbances in two generations of mice with incomplete penetrance to the F2 generation and alters the transcriptional profile of testis and sperm microRNA content. FASEB J 2013.
3. Campbell JM, Lane M, Vassiliev I, Nottle MB. Use of insulin to increase epiblast cell number: towards a new approach for improving ESC isolation from human embryos. Biomed Res Int 2013; 2013:150901.
4. Zander-Fox D, Cashman KS, Lane M. The presence of 1 mM glycine in vitrification solutions protects oocyte mitochondrial homeostasis and improves blastocyst development. J Assist Reprod Genet 2013; 30:107-116.
5. Campbell JM, Lane M, Vassiliev I, Nottle MB. Epiblast cell number and primary embryonic stem cell colony generation are increased by culture of cleavage stage embryos in insulin. J Reprod Dev 2013; 59:131-138.
9. Fullston T, Palmer NO, Owens JA, Mitchell M, Bakos HW, Lane M. Diet-induced paternal obesity in the absence of diabetes diminishes the reproductive health of two subsequent generations of mice. Hum Reprod 2012; 27:1391-1400.
10. Campbell JM, Nottle MB, Vassiliev I, Mitchell M, Lane M. Insulin increases epiblast cell number of in vitro cultured mouse embryos via the PI3K/GSK3/p53 pathway. Stem Cells Dev 2012; 21:2430-2441.
11. Palmer NO, Bakos HW, Owens JA, Setchell BP, Lane M. Diet and exercise in an obese mouse fed a high-fat diet improve metabolic health and reverse perturbed sperm function. Am J Physiol Endocrinol Metab 2012; 302:E768-780.
13. Owens JA, Fullston T, Mitchell M, Palmer N, Bakos H, Lane M. Diet Induced Paternal Obesity Impairs the Metabolic and Reproductive Health of Two Subsequent Generations. Journal of Developmental Origins of Health and Disease 2011; 2:S10-S10.
15. Zander-Fox DL, Lane M, Hamilton H. DAY4 VITRIFICATION FOLLOWING IN VITRO FERTILISATION (IVF) INCREASES CRYOPRESERVED EMBRYO PREGNANCY RATES COMPARED TO DAY5. Fertility and Sterility 2011; 96:S74-S74.
17. Gebhardt KM, Feil DK, Dunning KR, Lane M, Russell DL. Human cumulus cell gene expression as a biomarker of pregnancy outcome after single embryo transfer. Fertility and Sterility 2011; 96:47-U454.
18. Bakos HW, Henshaw RC, Mitchell M, Lane M. Paternal body mass index is associated with decreased blastocyst development and reduced live birth rates following assisted reproductive technology. Fertility and Sterility 2011; 95:1700-1704.
22. Fullston T, Mitchell M, Wakefield S, Lane M. Mitochondrial inhibition during preimplantation embryogenesis shifts the transcriptional profile of fetal mouse brain. Reproduction Fertility and Development 2011; 23:691-701.
26. Albuz FK, Sasseville M, Lane M, Armstrong DT, Thompson JG, Gilchrist RB. Simulated physiological oocyte maturation (SPOM): a novel in vitro maturation system that substantially improves embryo yield and pregnancy outcomes. Human Reproduction 2010; 25:2999-3011.
28. Wu LLY, Dunning KR, Yang X, Russell DL, Lane M, Norman RJ, Robker RL. High-Fat Diet Causes Lipotoxicity Responses in Cumulus-Oocyte Complexes and Decreased Fertilization Rates. Endocrinology 2010; 151:5438-5445.
29. Brown HM, Dunning KR, Robker RL, Boerboom D, Pritchard M, Lane M, Russell DL. ADAMTS1 Cleavage of Versican Mediates Essential Structural Remodeling of the Ovarian Follicle and Cumulus-Oocyte Matrix During Ovulation in Mice. Biology of Reproduction 2010; 83:549-557.
30. Fullston T, Mitchell M, Owens J, Palmer N, Bakos H, Lane M. Paternal Obesity Affects Male Offspring's Health and Sperm Function. Australian & New Zealand Journal of Obstetrics & Gynaecology 2010; 50:22-22.
31. Gebhardt K, Feil D, Lane M, Russell D. Predicting Pregnancy Success-Evaluation of Cumulus Cell Gene Expression Following Single Embryo Transfer. Australian & New Zealand Journal of Obstetrics & Gynaecology 2010; 50:23-23.
33. Gardner DK, Wale PL, Collins R, Lane M. Glucose Consumption by Human Embryos on Day 4 and Day 5 is Predictive of Pregnancy and Sex. Australian & New Zealand Journal of Obstetrics & Gynaecology 2010; 50:7-7.
34. Zander-Fox DL, Mitchell M, Thompson JG, Lane M. Alterations in mouse embryo intracellular pH by DMO during culture impair implantation and fetal growth. Reproductive Biomedicine Online 2010; 21:219-229.
35. Tremellen KP, Lane M. Avoidance of weekend oocyte retrievals during GnRH antagonist treatment by simple advancement or delay of hCG administration does not adversely affect IVF live birth outcomes. Human Reproduction 2010; 25:1219-1224.
36. Schelbach CJ, Kind KL, Lane M, Thompson JG. Mechanisms contributing to the reduced developmental competence of glucosamine-exposed mouse oocytes. Reproduction Fertility and Development 2010; 22:771-779.
37. Chin PY, Macpherson AM, Thompson JG, Lane M, Robertson SA. Stress response genes are suppressed in mouse preimplantation embryos by granulocyte-macrophage colony-stimulating factor (GM-CSF). Human Reproduction 2009; 24:2997-3009.
38. Hull ML, Nemeth D, Hague WM, Wilkinson C, Liebelt J, Lane M, Feil D. Mitochondrial fatty acid transport enzyme deficiency-implications for in vitro fertilization. Fertility and Sterility 2009; 91.
39. Robker RL, Akison LK, Bennett BD, Thrupp PN, Chura LR, Russell DL, Lane M, Norman RJ. Obese Women Exhibit Differences in Ovarian Metabolites, Hormones, and Gene Expression Compared with Moderate-Weight Women. Journal of Clinical Endocrinology & Metabolism 2009; 94:1533-1540.
40. Yeo CX, Gilchrist RB, Lane M. Disruption of Bidirectional Oocyte-Cumulus Paracrine Signaling During In Vitro Maturation Reduces Subsequent Mouse Oocyte Developmental Competence. Biology of Reproduction 2009; 80:1072-1080.
41. Mitchell M, Schulz SL, Armstrong DT, Lane M. Metabolic and Mitochondrial Dysfunction in Early Mouse Embryos Following Maternal Dietary Protein Intervention. Biology of Reproduction 2009; 80:622-630.
42. Mitchell M, Cashman KS, Gardner DK, Thompson JG, Lane M. Disruption of Mitochondrial Malate-Aspartate Shuttle Activity in Mouse Blastocysts Impairs Viability and Fetal Growth. Biology of Reproduction 2009; 80:295-301.
43. Watkinson RM, Herkenne C, Guy RH, Hadgraft J, Oliveira G, Lane ME. Influence of Ethanol on the Solubility, Ionization and Permeation Characteristics of Ibuprofen in Silicone and Human Skin. Skin Pharmacology and Physiology 2009; 22:15-21.
44. Lopes AS, Dalvit GC, Lane M, Thompson JG. THE PEAK OF OXYGEN CONSUMPTION AT THE TIME OF SPERM PENETRATION IS ASSOCIATED WITH AN INCREASE IN REACTIVE OXYGEN SPECIES PRODUCTION IN BOVINE ZYGOTES. Reproduction Fertility and Development 2009; 21:159-159.
45. Fullston T, Mitchell M, Wakefield S, Filby A, Lane M. Micro-Array Analysis of Fetal Mouse Brain Following Induction of Mitochondrial Dysfunction During Pre-Implantation Embryo Development. Biology of Reproduction 2009:102-102.
47. Lekamge DN, Lane M, Gilchrist RB, Tremellen KP. Increased gonadotrophin stimulation does not improve IVF outcomes in patients with predicted poor ovarian reserve. Journal of Assisted Reproduction and Genetics 2008; 25:515-521.
51. Wakefield SL, Lane M, Schulz SJ, Hebart ML, Thompson JG, Mitchell M. Maternal supply of omega-3 polyunsaturated fatty acids alter mechanisms involved in oocyte and early embryo development in the mouse. American Journal of Physiology-Endocrinology and Metabolism 2008; 294:E425-E434.
52. Yeo CX, Gilchrist RB, Thompson JG, Lane M. Exogenous growth differentiation factor 9 in oocyte maturation media enhances subsequent embryo development and fetal viability in mice. Human Reproduction 2008; 23:67-73.
60. Bakos HW, Froiland DA, Thompson JG, Lane M. Elevated glucose levels induce lipid peroxidation and DNA damage in human spermatozoa. Australian & New Zealand Journal of Obstetrics & Gynaecology 2007; 47:A1-A1.
64. Banwell KM, Lane M, Russell DL, Kind KL, Thompson JG. Oxygen concentration during in vitro maturation of murine oocytes influences subsequent fetal and placental outcomes. Reproduction Fertility and Development 2007; 19:285-285.
68. Sheehan CB, Lane M, Gardner DK. The CryoLoop facilitates re-vitrification of embryos at four successive stages of development without impairing embryo growth. Human Reproduction 2006; 21:2978-2984.
69. Kelley RL, Kind KL, Lane M, Robker RL, Thompson JG, Edwards LJ. Recombinant human follicle-stimulating hormone alters maternal ovarian hormone concentrations and the uterus and perturbs fetal development in mice. American Journal of Physiology-Endocrinology and Metabolism 2006; 291:E761-E770.
70. Thompson J, Lane M. Reply: In unselected patients, elective single embryo transfer prevents all multiples, but results in significantly lower pregnancy rates compared to double embryo transfer. Human Reproduction 2006; 21:2195-2195.
73. Sutton-McDowall ML, Mitchell M, Cetica P, Dalvit G, Pantaleon M, Lane M, Gilchrist RB, Thompson JG. Glucosamine supplementation during in vitro maturation inhibits subsequent embryo development: Possible role of the of developmental competence. Biology of Reproduction 2006; 74:881-888.
74. Feil D, Lane M, Roberts CT, Kelley RL, Edwards LJ, Thompson JG, Kind KL. Effect of culturing mouse embryos under different oxygen concentrations on subsequent fetal and placental development. Journal of Physiology-London 2006; 572:87-96.
76. Zander DL, Thompson JG, Lane M. Perturbations in mouse embryo development and viability caused by ammonium are more severe after exposure at the cleavage stages. Biology of Reproduction 2006; 74:288-294.
77. Herrick JR, Lane M, Gardner DK, Behboodi E, Memili E, Blash S, Echelard Y, Krisher RL. Metabolism, protein content, and in vitro embryonic development of goat cumulus-oocyte complexes matured with physiological concentrations of glucose and L-lactate. Molecular Reproduction and Development 2006; 73:256-266.
78. Thompson J, Lane M, Robertson S. Adaptive responses of early embryos to their microenvironment and consequences for post-implantation development. Early Life Origins of Health and Disease 2006; 573:58-69.
79. Yeo CX, Gilchrist RB, Thompson JG, Lane M. 6-Phosphofructo-2-kinase/fructose-2, 6-bisphosphatase (PFKFB) is present in mouse cumulus oocyte complexes and regulated by growth differentiation factor 9 (GDF9). Biology of Reproduction 2006:104-104.
82. Mitchell M, Schulz SJ, Hamon R, Armstrong DT, Lane M. The influence of maternal dietary protein supply on oocyte quality and mitochondrial homeostasis of in vivo and in vitro produced mouse embryos. Biology of Reproduction 2006:91-91.
86. Karagenc L, Lane M, Gardner DK. Granulocyte-macrophage colony-stimulating factor stimulates mouse blastocyst inner cell mass development only when media lack human serum albumin. Reproductive Biomedicine Online 2005; 10:511-518.
90. Karagenc L, Lane M, Gardner DK. Oestradiol, cyclodextrin-encapsulated 17 beta-oestradiol and the oestradiol solubilizer 2-hydroxypropyl-beta-cyclodextrin all impair preimplantation mouse embryo development. Reproductive Biomedicine Online 2004; 9:280-286.
91. Thompson JG, Feil D, Edwards LJ, Lane M, Kind KL. Mouse fetal development is perturbed by culture in low oxygen concentration that increases expression of oxygen-sensitive genes via hypoxia inducible factors: A non-epigenetic embryonic programming phenomenon? Biology of Reproduction 2004:218-218.
92. Lane M, Gardner DK. Ammonium induces aberrant blastocyst differentiation, metabolism, pH regulation, gene expression and subsequently alters fetal development in the mouse. Biology of Reproduction 2003; 69:1109-1117.
95. Lane M, Gardner DK, Hasler MJ, Hasler JF. Use of G1.2/G2.2 media for commercial bovine embryo culture: equivalent development and pregnancy rates compared to co-culture. Theriogenology 2003; 60:407-419.
97. Gardner DK, Stilley KS, Lane M. High protein diet inhibits inner cell mass formation and increases apoptosis in mouse blastocysts developed in vivo by increasing ammonium in the female tract. Pediatric Research 2003; 53:47A-47A.
98. Gardner DK, Lane M, Stevens J, Schoolcraft WB. Changing the start temperature and cooling rate in a slow-freezing protocol increases human blastocyst viability. Fertility and Sterility 2003; 79:407-410.
99. Lane M, Maybach JM, Hooper K, Hasler JF, Gardner DK. Cryo-survival and development of bovine blastocysts are enhanced by culture with recombinant albumin and hyaluronan. Molecular Reproduction and Development 2003; 64:70-78.
102. Karagenc L, Lane M, Gardner DK. Granulocyte macrophage colony stimulating factor (GM-CSF) stimulates mouse blastocyst ICM development only when culture conditions are suboptimal. Fertility and Sterility 2002; 78:S170-S170.
103. Karagenc L, Lane M, Gardner DK. Estradiol, cyclodextrin-encapsulated 17 beta-estradiol and the estradiol solubilizer 2-hydroxypropyl-beta-cyclodextrin impair preimplantation mouse embryo development. Fertility and Sterility 2002; 78:S276-S276.
104. Schoolcraft W, Lane M, Stevens J, Gardner DK. Increased hyaluronan concentration in the embryo transfer medium results in a significant increase in human embryo implantation rate. Fertility and Sterility 2002; 78:S5-S5.
110. Gardner DK, Lane M, Stevens J, Schoolcraft WB. Increased human blastocyst viability by changing the start temperature and cooling rate in a slow freezing protocol. Fertility and Sterility 2001; 76:S80-S80.
111. Steeves CL, Lane M, Bavister BD, Phillips KP, Baltz JM. Differences in intracellular pH regulation by Na+/H+ antiporter among two-cell mouse embryos derived from females of different strains. Biology of Reproduction 2001; 65:14-22.
115. Gandhi AP, Lane M, Gardner DK, Krisher RL. Substrate utilization in porcine embryos cultured in NCSU23 and G1.2/G2.2 sequential culture media. Molecular Reproduction and Development 2001; 58:269-275.
120. Winger QA, Lane M, Gardner DK. Expression of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase mRNA isoforms in mouse oocytes and embryos developed in vivo and in vitro. Biology of Reproduction 2001; 64:178-179.
124. Gardner DK, Lane MW, Lane M. EDTA stimulates cleavage stage bovine embryo development in culture but inhibits blastocyst development and differentiation. Molecular Reproduction and Development 2000; 57:256-261.
125. Gardner DK, Lane M, Stevens J, Schlenker T, Schoolcraft WB. Blastocyst score affects implantation and pregnancy outcome: towards a single blastocyst transfer. Fertility and Sterility 2000; 73:1155-1158.
134. Hall-Woods ML, Krisher RL, Lane M, Gardner DK, Asa CS. Comparison of Gardner's G1/G2 sequential media and buffalo rat liver (BRL) cell go-culture for bovine in-vitro embryo production. Biology of Reproduction 2000; 62:316-316.
137. Lane M, Ludwig TE, Bavister BD. Phosphate induced developmental arrest of hamster two-cell embryos is associated with disrupted ionic homeostasis. Molecular Reproduction and Development 1999; 54:410-417.
138. Lane M, Bavister BD, Lyons EA, Forest KT. Containerless vitrification of mammalian oocytes and embryos - Adapting a proven method for flash-cooling protein crystals to the cryopreservation of live cells. Nature Biotechnology 1999; 17:1234-1236.
139. Schoolcraft WB, Gardner DK, Lane M, Schlenker T, Hamilton F, Meldrum DR. Blastocyst culture and transfer: analysis of results and parameters affecting outcome in two in vitro fertilization programs. Fertility and Sterility 1999; 72:604-609.
140. Gardner DK, Rodriegez-Martinez H, Lane M. Fetal development after transfer is increased by replacing protein with the glycosaminoglycan hyaluronan for mouse embryo culture and transfer. Human Reproduction 1999; 14:2575-2580.
149. Lane M, Boatman DE, Albrecht RM, Bavister BD. Intracellular divalent cation homeostasis and developmental competence in the hamster preimplantation embryo. Molecular Reproduction and Development 1998; 50:443-450.
152. Gardner DK, Vella P, Lane M, Wagley L, Schlenker T, Schoolcraft WB. Culture and transfer of human blastocysts increases implantation rates and reduces the need for multiple embryo transfers. Fertility and Sterility 1998; 69:84-88.
158. Lane M, Gardner DK. Nonessential amino acids and glutamine decrease the time of the first three cleavage divisions and increase compaction of mouse zygotes in vitro. Journal of Assisted Reproduction and Genetics 1997; 14:398-403.
161. Gardner DK, Lane M, RodriguezMartinez H. Mouse fetal development after transfer is increased by replacing protein with the glycosaminoglycan hyaluronate for embryo culture. Human Reproduction 1997; 12:O215-O215.
165. Gardner DK, Lane M. Alleviation of the 2-cell block in CF1 mouse embryos is associated with an increase in the ATP:ADP ratio and subsequent inhibition of phosphofructokinase. Biology of Reproduction 1997; 56:216-216.
167. Gardner DK, Vella P, Lane M, Wagley L, Schlenker T, Schoolcraft WB. Culture and transfer of human blastocysts increases implantation rates and reduces the need for multiple embryo transfers. Fertility and Sterility 1997:O002-O002.
168. Gardner DK, Lane M. Alleviation of the '2-cell block' and development to the blastocyst of CF1 mouse embryos: Role of amino acids, EDTA and physical parameters. Human Reproduction 1996; 11:2703-2712.
171. Gardner DK, Lane M, Calderon I, Leeton J. Environment of the preimplantation human embryo in vivo: Metabolite analysis of oviduct and uterine fluids and metabolism of cumulus cells. Fertility and Sterility 1996; 65:349-353.
175. Lane M, Gardner DK. INCREASE IN POSTIMPLANTATION DEVELOPMENT OF CULTURED MOUSE EMBRYOS BY AMINO-ACIDS AND INDUCTION OF FETAL RETARDATION AND EXENCEPHALY AMMONIUM-IONS. Journal of Reproduction and Fertility 1994; 102:305-312.
176. Gardner DK, Lane M, Spitzer A, Batt PA. ENHANCED RATES OF CLEAVAGE AND DEVELOPMENT FOR SHEEP ZYGOTES CULTURED TO THE BLASTOCYST STAGE IN-VITRO IN THE ABSENCE OF SERUM AND SOMATIC-CELLS - AMINO-ACIDS, VITAMINS, AND CULTURING EMBRYOS IN GROUPS STIMULATE DEVELOPMENT. Biology of Reproduction 1994; 50:390-400.
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