Dr Steven Polyak

Biography/ Background

2001 - current Research Fellow, School of Molecular and Biomedical Science, University of Adelaide

1999 - 2001 Research Associate, Hanson Institute

2000 PhD University of Adelaide

Publications

Refereed Journal Articles

Pendini, N.R., Polyak, S.W., Booker, G.W., Wallace, J.C. & Wilce, M.C.J. (2008) Purification, crystallisation and preliminary crystallographic analysis of biotin protein ligase from Staphylococcus aureus Acta Crystallogr Sect F Struct Biol Cryst Commun. 64(Pt 6):520-3

Pendini, N.R., Bailey, L.M., Booker, G.W., Wilce, M., Wallace, J.C. & Polyak, S.W. (2008) Microbial relatives of biotin protein ligase aid in understanding holocarboxylase synthetase deficiency BBA-Proteins and proteomics 784(7-8):973-82

Bailey, L. M., Ivanov, R., Jitrapakdee, S., Wilson, C. J., Wallace, J. C. & Polyak, S. W. (2008) Reduced half-life of holocarboxylase synthetase from patients with severe mutiple carboxylase deficiency Human Mutation Mutation in Brief #1009, 29:E47-57

Ng, B., Polyak, S. W., Bird, D., Bailey, L. M., Wallace, J. C. & Booker, G. W. (2008) Escherichia coli biotin protein ligase: Characterisation and development of a high-throughput assay Anal. Biochem. 376(1): 131-6

Bailey, L. M., Ivanov, R., Wallace, J. C. & Polyak, S. W. (2008) Artifactual detection of biotin on histones by streptavidin Anal. Biochem. 373(1):71-7

Jitrapakdee, S., Surinya, K., Adina-Zada, A., Polyak, S. W., Stojkoski, C., Smyth, R., Booker, G. W., Cleland, C. C., Attwood, P. & Wallace, J. C. (2007) Conserved Glu40 and Glu433
of the biotin carboxylase domain of yeast pyruvate carboxylase I isoenzyme are essential for the association of tetramers Int. J. Biochem. Cell Biol. 39(11):2120-34

Coles, L. S., Bartley, M. A., Bert, A., Hunter J., Polyak, S., Diamond, P., Vadas, M. A. & Goodall, G. J. (2004) A multi-protein complex containing cold shock domain (Y-box) and polypyrmidine tract binding protein binding proteins forms on the vascular endothelial growth factor mRNA – Role in mRNA stabilisation Eur J. Biochem. 271(3):648-60

D. L. Johnson, S. W. Polyak, J. C. Wallace and L. L. Martin (2003) Thioredoxin Electrochemistry. Lett. In Pept. Sci. 10, 495-500

Polyak, S. W., Chapman-Smith, A., Mulhern, T. D., Cronan, J. E. Jnr. & Wallace, J. C. (2001) Mutational analysis of protein substrate presentation in the post-translational attachment of biotin to biotin domains. J. Biol. Chem. 276 (5): 3037-3045

Dibbens, J. A., Polyak, S. W., Risau, A., Vadas, M. A. & Goodall, G. J. (2001) Nucleotide sequence of the mouse VEGF 3’ UTR and quantitative analysis of sites of polyadenylation Biochim. Biophys. Acta 1518 : 57-62

Polyak, S. W., Chapman-Smith, A., Brautigan, P. & Wallace, J. C. (1999) Biotin protein ligase from Saccharomyces cerevisiae: the N-terminal domain is required for full activity J. Biol. Chem. 274 (46) : 32847-32854

Polyak, S.W., Forsberg, G., Forbes, B.E., McNeil, K.A., Aplin, S. & Wallace J.C (1997) Introduction of spacer peptides N-terminal to a cleavage recognition motif in recombinant fusion proteins can improve site specific cleavage Prot. Eng. 10 (6) : 615 –619

Invited book chapter

Polyak, S. W. & Chapman-Smith, A. (2004) Biotin Encyclopaedia Of Biological Chemistry [Ed. Lennarz, W. and Lane, M. D.] 1 : 174-178

Patents

Polyak, S. W., Wallace, J.C., Stojkoski, C and Booker, GW (2005) Inhibitors of biotin protein ligase. PCT WO 2006056007AU

Pendini, N.R., Polyak, S. W., Wallace, J.C., Booker, G.W. & Wilce, M. (2007) Crystal structure of a bacterial enzyme Prov. Patent Application 2007906228 filed 13 November 2007

Professional Associations

Australian Society for Medical Research
Australian Society for Biochemistry and Molecular Biology

Entry last updated: Tuesday, 26 May 2009