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Dr Steven Polyak

Telephone +61 8 8313 6042
Position Research Associate
Email steven.polyak@adelaide.edu.au
Fax +61 8 8313 4362
Building Molecular Life Sciences
Floor/Room 2 06
Campus North Terrace
Org Unit School of Molecular and Biomedical Science

To link to this page, please use the following URL:
http://www.adelaide.edu.au/directory/steven.polyak

Biography/ Background

2001 - current Research Fellow, School of Molecular and Biomedical Science, University of Adelaide

1999 - 2001 Research Associate, Hanson Institute

2000 PhD University of Adelaide

Teaching Interests

Lecturing Courses

Biomedical Science IIB

Masters Biotechnology

Research Interests

Protein structure and function         

Small molecule drug discovery

Biotin (vitamin B7 or H)

Antibiotics

 

Publications

Refereed Journal Articles

Publications list

 

Click here for updated publication list

Selected Papers

Paparella, A.P., Soares da Costa, T.P., Yap, M.Y., Tieu, W., Wilce, M.C.J., Booker, G.W., Abell, A.D. & Polyak, S.W. (2014) Structure guided design of biotin protein ligase inhibitors for antibiotic discovery. Curr. Top. Med. Chem., 14(1): 4-20

Soares da Costa, T.P., Yap, M.Y., Perugini. M.A., Wallace, J.C., Abell, A.D., Wilce, M.C.J., Polyak, S.W. & Booker, G.W. (2014) Dual roles for F123 in protein homodimerization and inhibitor binding to biotin protein ligase from Staphylococcus aureus. Mol Microbiol. 91(1):110-120.

Tieu, W., Soares Da Costa, T.P., Yap, M.Y., Keeling, K.L., Wilce, M.C.J., Wallace, J.C., Booker, G.W., Polyak, S. W. & Abell, A.D. (2013) Optimising in situ click chemistry: the screening and identification of biotin protein ligase inhibitors. Chem. Sci. 4, 3533-353.

Pendini, N.R., Yap, M.Y., Polyak, S. W., Cowieson, N.P., Booker, G.W., Wallace, J.C., Abell, A.D., Wilce, J.A. & Wilce, M.C.J. (2013) Structural characterisation of Staphylocococcus aureus biotin protein ligase: An antibiotic target. Prot. Sci. 22(6): 762-73

Soares da Costa, T.P. Tieu, W., Yap, M.Y., Zvarec, O., Bell, J., Turnidge, J.D., Wallace. J.C., Booker, G.W., Wilce, M.C.J., Abell, A.D., Polyak, S. W. (2012) Biotin analogues with antibacterial activity are potent inhibitors of biotin protein ligase. ACS Med. Chem. Letts.

Soares da Costa, T.P., Tieu, W., Yap, M.Y., Pendini, N.R., Polyak, S. W., Sejer Pedersen, D., Morona, R. Turnidge, J.D., Wallace, J.C. Wilce, M.C.J., Booker, G.W. & Abell, A.D. (2012) Selective inhibition of Biotin Protein Ligase from Staphylococcus aureus. J. Biol. Chem. 287(21):17823-32.

Mayende, L., Swift, R.D., Bailey, L.M., Soares da Costa, T.P., Wallace, J.C., Booker, G.W. & Polyak, S. W. (2012) A novel molecular mechanism to explain biotin-unresponsive Holocarboxylase synthetase. J Mol. Med. 90(1):81-88

Polyak, S. W., Abell, A.D., Wilce, M.C.J., Zhang, L. & Booker, G.W. (2012) Structure, function and selective inhibition of bacterial acetyl CoA carboxylase. Appl. Micro. Biotech. 

Salaemae, W., Azhar, A., Booker, G.W. & Polyak, S. W. (2011) Biotin biosynthesis in Mycobacterium tuberculosis: Physiology, biochemistry and molecular intervention. Protein & Cell. 2(9):691-5

Bailey, L. M., Wallace, J.C. & Polyak, S. W. (2010) Holocarboxylase synthetase: Correlation of protein localisation with biological function Arch Biochem Biophys 496(1):45-52

Pendini, N.R., Bailey, L.M., Booker, G.W., Wilce, M., Wallace, J.C. & Polyak, S. W. (2008) Microbial relatives of biotin protein ligase aid in understanding holocarboxylase synthetase deficiency BBA-Proteins and proteomics 784(7-8):973-82

Bailey, L.M., Ivanov,R., Jitrapakdee, S., Wilson, C.J., Wallace, J.C. & Polyak, S. W. (2008) Reduced half-life of holocarboxylase synthetase from patients with severe mutiple carboxylase deficiency Human Mutation Mutation in Brief #1009, 29:E47-57

Ng, B., Polyak, S. W., Bird, D., Bailey, L.M., Wallace, J.C. & Booker, G.W. (2008) Escherichia coli biotin protein ligase: Characterisation and development of a high-throughput assay Anal. Biochem. 376(1): 131-6

Bailey, L.M., Ivanov, R., Wallace, J.C. & Polyak, S. W. (2008) Artifactual detection of biotin on histones by streptavidin Anal. Biochem. 373(1):71-7

Jitrapakdee, S., Surinya, K., Adina-Zada, A., Polyak, S. W., Stojkoski, C., Smyth, R., Booker, G.W., Cleland, C.C., Attwood, P. & Wallace, J.C. (2007) Conserved Glu40 and Glu433
of the biotin carboxylase domain of yeast pyruvate carboxylase I isoenzyme are essential for the association of tetramers Int. J. Biochem. Cell Biol. 39(11):2120-34

Polyak, S. W., Chapman-Smith, A., Mulhern, T.D., Cronan, J.E. Jnr. & Wallace, J.C. (2001) Mutational analysis of protein substrate presentation in the post-translational attachment of biotin to biotin domains. J. Biol. Chem. 276 (5): 3037-3045

Polyak, S. W., Chapman-Smith, A., Brautigan, P. & Wallace, J. C. (1999) Biotin protein ligase from Saccharomyces cerevisiae: the N-terminal domain is required for full activity J. Biol. Chem. 274 (46): 32847-32854

Polyak, S. W., Forsberg, G., Forbes, B.E., McNeil, K.A., Aplin, S. & Wallace J.C (1997) Introduction of spacer peptides N-terminal to a cleavage recognition motif in recombinant fusion proteins can improve site specific cleavage Prot. Eng. 10 (6) : 615 –619

 

Invited book chapters

Polyak, S. W. & Chapman-Smith, A. (2013) Biotin In Encyclopaedia of Biological Chemistry, 2nd edition. Eds Lennarz, W.J. and Lane, M.D. pp 221-225 [ISBN 978-0-12-378631-9] (http://dx.doi.org/10.1016/B978-0-12-378630-2.00005-0).

Polyak, S. W., Bailey, L.M., Azhar, A. & Booker, G.W. (2012) Biotin (Vitamin H or B7) In Micronutrients: Sources, properties and health benefits. Eds Betancourt, A.I. & Gaitan, H.F. 65-93 [ISBN 978-1-62100-834-7].


Patents

Polyak, S.W., Tieu, W., Soares da Costa, T.P., Jarrad, A., Yap, M.Y., Pendini, N.R., Morona, R., Wallace, J.C. Wilce, M.C.J., Booker, G.W. & Abell, A.D. Novel AntiMicrobial Compounds, PCT/AU2012/001138, filed 21 September 2012. 

Polyak, S. W., Wallace, J.C., Stojkoski, C and Booker, GW (2005) Inhibitors of biotin protein ligase. PCT WO 2006056007AU

Pendini, N.R., Polyak, S. W., Wallace, J.C., Booker, G.W. & Wilce, M. (2007) Crystal structure of a bacterial enzyme Prov. Patent Application 2007906228 filed 13 November 2007

Professional Associations

Executive Director, Australian Society for Medical Research

Convener, 6th Australian Health and Medical Research Congress 2012

Australian Society for Biochemistry and Molecular Biology

Entry last updated: Wednesday, 13 Aug 2014

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