New publication in Scientific Reports

The Centre team's latest publication in Scientific Reports pits light sheet against confocal microscopy and quantifies the impact of volumetric imaging on DNA integrity in preimplantation mouse embryos.

For the first time, the publication showed that when compared to confocal microscopy (most commonly used imaging system in biology), light sheet microscopy is associated with:

• 10-fold faster image acquisition
• Low photobleaching rate
• Null impact on levels of DNA damage following volumetric imaging

The paper also showed that light sheet requires at least more than one round of volumetric imaging to achieve detectable levels of DNA damage.

Read the paper here: https://www.nature.com/articles/s41598-024-71443-x

Authors: Darren Chow, Erik Schartner, Stella Corsetti, Avinash Upadhya, Joséphine Morizet, Frank Gunn-Moore, Kylie Dunning, Kishan Dholakia.