Debaryomyces hansenii

Synonymy: 
Candida famata.

Debaryomyces hansenii is a common environmental isolate.

It may be isolated from human skin, and is only rarely recovered from blood stream infections.

RG-1 organism.

Culture: 
Colonies (SDA) white to cream-coloured smooth, glabrous, yeast-like.

Microscopy: 
Ovoid to broadly ellipsoidal budding blastoconidia, 3.5-5 x 2-3.5 µm in size. No pseudohyphae produced. Asci when present are spherical, persistent, containing one to two spherical ascospores with rough walls.

India ink preparation: 
Negative - no capsules present.

Dalmau plate culture: 
Spherical to ovoid budding yeast cells only. Pseudohyphae are usually lacking.

Molecular identification: 
ITS sequencing recommended.

MALDI-TOF MS: 
Able to accurately identify this species.

Physiological Tests: + Positive, - Negative, v Variable, w Weak, s Slow, nd No Data
Germ Tube - L-Sorbose v L-Arabinose +,w D-Glucitol +,w
Fermentation   Sucrose + D-Arabinose v 𝝰-M-D-Glucoside +,w
Glucose -,w Maltose + D-Ribose v D-Gluconate +,w
Galactose -,w Cellobiose + L-Rhamnose v DL-Lactate v
Sucrose -,w Trehalose + D-Glucosamime v myo-Inositol -
Maltose -,w Lactose v N-A-D-glucosamine v 2-K-D-Gluconate +
Lactose - Melibiose v Glycerol + D-Glucuronate v
Trehalose -,w Raffinose + Erythritol v Nitrate -
Assimilation   Melezitose v Ribitol + Urease -
Glucose + Soluble Starch v Galactitol v 0.1% Cycloheximide v
Galactose + D-Xylose + D-Mannitol + Growth at 40C -

Key features: 
Germ tube negative yeast and sugar assimilation pattern.

Antifungal susceptibility: Debaryomyces hansenii (Diekema et al. 2009, Beyda et al. 2013, Espinel-Ingroff et al. 2014 and Australian national data); MIC µg/mL.

Antifungal No ≤0.03 0.06 0.125 0.25 0.5 1 2 4 8 16 32 ≥64
AMB 27   1 3 7 13 2 1          
FLU 76     9 18 13 9 8 11 2 3   3
VORI 79 46 16 9 4   2 1 1        
POSA 72 19 20 12 8 8 2 3          
ITRA 11 2   1 3 2 1       2    
ANID 23 1 2 1 1   8 10          
MICA 23 4 1   2 8 5 3          
CAS 26 1 2 3 8 5 4 1     2    
5FC 11 1 5 1 1 1             2

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