Clavispora lusitaniae
Synonymy:
Candida lusitaniae
Clavispora lusitaniae is a known cause of disseminated candidiasis, including septicaemia and pyelonephritis.
C. lusitaniae was first isolated from the digestive tract of warm-blooded animals and environmental isolations have been made from cornmeal, citrus peel, fruit juices, and milk from cows with mastitis.
RG-2 organism.
Culture:
Colonies (SDA) white to cream-coloured smooth, glabrous, yeast-like.
Microscopy:
Ovoid to ellipsoidal budding blastoconidia, 1.5-6.0 x 2.5-10µm.
India Ink Preparation:
Negative - no capsules present.
Dalmau Plate Culture:
Abundant pseudohyphae with short chains of blastoconidia.
Molecular Identification:
ITS sequencing recommended.
MALDI-TOF MS:
Able to accurately identify this species.
Physiological Tests: + Positive, - Negative, v Variable, w Weak, s Slow, nd No Data | |||||||
---|---|---|---|---|---|---|---|
Germ Tube | - | L-Sorbose | + | L-Arabinose | v | D-Glucitol | + |
Fermentation | Sucrose | + | D-Arabinose | - | 𝝰-M-D-Glucoside | v | |
Glucose | + | Maltose | + | D-Ribose | - | D-Gluconate | s |
Galactose | v | Cellobiose | + | L-Rhamnose | v | DL-Lactate | +,w |
Sucrose | v | Trehalose | + | D-Glucosamime | - | myo-Inositol | - |
Maltose | v | Lactose | - | N-A-D-glucosamine | + | 2-K-D-Gluconate | + |
Lactose | - | Melibiose | - | Glycerol | + | D-Glucuronate | nd |
Trehalose | v | Raffinose | - | Erythritol | - | Nitrate | - |
Assimilation | Melezitose | + | Ribitol | s | Urease | - | |
Glucose | + | Soluble Starch | - | Galactitol | - | 0.1% Cycloheximide | - |
Galactose | + | D-Xylose | + | D-Mannitol | + | Growth at 40C | + |
Key Features:
Germ tube negative yeast and sugar assimilation pattern. Note: C. lusitaniae may also be difficult to distinguish from Candida tropicalis using some yeast identification systems.
Antifungal | No | ≤0.016 | 0.03 | 0.06 | 0.125 | 0.25 | 0.5 | 1 | 2 | 4 | 8 | 16 | 32 | ≥64 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
AMB | 273 | 1 | 9 | 34 | 99 | 102 | 20 | 6 | 2 | |||||
FLU | 355 | 13 | 38 | 86 | 106 | 52 | 32 | 11 | 4 | 2 | 5 | 6 | ||
ISAV | 50 | 17 | 20 | 10 | 3 | |||||||||
VORI | 349 | 291 | 25 | 16 | 4 | 4 | 6 | 2 | ∫ | |||||
POSA | 326 | 59 | 108 | 111 | 26 | 16 | 2 | 4 | ||||||
ITRA | 169 | 3 | 6 | 47 | 61 | 28 | 19 | 4 | 1 | |||||
ANID | 247 | 2 | 15 | 65 | 85 | 78 | 16 | 2 | ||||||
MICA | 275 | 3 | 22 | 60 | 78 | 60 | 16 | 7 | 1 | |||||
CAS | 247 | 2 | 4 | 12 | 86 | 91 | 38 | 12 | 1 | 1 | ||||
5FC | 275 | 15 | 77 | 22 | 54 | 67 | 20 | 5 | 3 | 2 | 1 | 5 | 4 |