Debaryomyces hansenii

Synonymy:
Candida famata.

Debaryomyces hansenii is a common environmental isolate.

It may be isolated from human skin, and is only rarely recovered from blood stream infections.

RG-1 organism.

Culture:
Colonies (SDA) white to cream-coloured smooth, glabrous, yeast-like.

Microscopy:
Ovoid to broadly ellipsoidal budding blastoconidia, 3.5-5 x 2-3.5 µm in size. No pseudohyphae produced. Asci when present are spherical, persistent, containing one to two spherical ascospores with rough walls.

India ink preparation:
Negative - no capsules present.

Dalmau plate culture:
Spherical to ovoid budding yeast cells only. Pseudohyphae are usually lacking.

Molecular identification:
ITS sequencing recommended.

MALDI-TOF MS:
Able to accurately identify this species.

Physiological Tests: + Positive, - Negative, v Variable, w Weak, s Slow, nd No Data
Germ Tube	-	L-Sorbose	v	L-Arabinose	+,w	D-Glucitol	+,w
Fermentation		Sucrose	+	D-Arabinose	v	𝝰-M-D-Glucoside	+,w
Glucose	-,w	Maltose	+	D-Ribose	v	D-Gluconate	+,w
Galactose	-,w	Cellobiose	+	L-Rhamnose	v	DL-Lactate	v
Sucrose	-,w	Trehalose	+	D-Glucosamime	v	myo-Inositol	-
Maltose	-,w	Lactose	v	N-A-D-glucosamine	v	2-K-D-Gluconate	+
Lactose	-	Melibiose	v	Glycerol	+	D-Glucuronate	v
Trehalose	-,w	Raffinose	+	Erythritol	v	Nitrate	-
Assimilation		Melezitose	v	Ribitol	+	Urease	-
Glucose	+	Soluble Starch	v	Galactitol	v	0.1% Cycloheximide	v
Galactose	+	D-Xylose	+	D-Mannitol	+	Growth at 40C	-

Key features:
Germ tube negative yeast and sugar assimilation pattern.

Antifungal susceptibility: *Debaryomyces hansenii* (Diekema *et al.* 2009, Beyda *et al.* 2013, Espinel-Ingroff et al. 2014 and Australian national data); **MIC µg/mL.**
Antifungal	No	≤0.03	0.06	0.125	0.25	0.5	1	2	4	8	16	≥64
AMB	29		2	3	7	14	2	1
FLU	78			9	18	14	9	9	11	2	3	3
VORI	81	48	16	9	4		2	1	1
POSA	75	20	22	10	8	10	2	3
ITRA	14	2	2	1	4	3					2
ANID	26	1	2	3	1		9	10
MICA	25	5	1		2	8	6	3
5FC	14	1	7	1	1	2						2

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