Torulaspora delbrueckii

Synonymy:
Candida colliculosa

Torulaspora delbrueckii is a rare cause of candidaemia.

RG-1 organism.

Culture:
Colonies (SDA) white to cream-coloured smooth, glabrous, yeast-like.

Microscopy:
Spherical to ellipsoidal budding blastoconidia, 2-6 x 3-7 µm in size. Ascospores may be produced on 5% malt extract or cornmeal agar after 5-30 days at 25C.

India ink preparation:
Negative - no capsules present.

Dalmau plate culture:
Budding yeast cells only. No pseudohyphae or true hyphae produced.

Molecular identification:
ITS sequencing recommended.

MALDI-TOF MS:
Able to accurately identify this species.

Physiological Tests: + Positive, - Negative, v Variable, w Weak, s Slow, nd No Data
Germ Tube	-	L-Sorbose	v	L-Arabinose	-	D-Glucitol	v
Fermentation		Sucrose	v	D-Arabinose	-	𝝰-M-D-Glucoside	v
Glucose	+	Maltose	v	D-Ribose	-	D-Gluconate	v
Galactose	v	Cellobiose	-	L-Rhamnose	-	DL-Lactate	v
Sucrose	v	Trehalose	-,s	D-Glucosamime	-	myo-Inositol	-
Maltose	v	Lactose	-	N-A-D-glucosamine	-	2-K-D-Gluconate	+
Lactose	-	Melibiose	-	Glycerol	v	D-Glucuronate	v
Trehalose	v	Raffinose	v	Erythritol	-	Nitrate	-
Assimilation		Melezitose	v	Ribitol	v	Urease	-
Glucose	+	Soluble Starch	-	Galactitol	-	0.1% Cycloheximide	-
Galactose	v	D-Xylose	v	D-Mannitol	v	Growth at 37C	v

Key features:
Asci containing one to four spheroidal ascospores, strong growth at 37C and a variable sugar assimilation profile.

Antifungal susceptibility: *Torulaspora delbrueckii* very limited data (Australian national data); **MIC µg/mL.**
Antifungal	No	≤0.03	0.06	0.125	0.5	4
AMB	1				1
FLU	1					1
VORI	1		1
POSA	1			1
ITRA	1			1
ANID	1	1
MICA	1	1
5FC	1			1

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