Yarrowia lipolytica
Synonymy:
Candida lipolytica
Yarrowia lipolytica is a rare cause of candidaemia.
RG-1 organism.
Culture:
Colonies (SDA) white to cream-coloured smooth, glabrous, yeast-like.
Microscopy:
Spherical, ellipsoidal to elongate budding blastoconidia, 3-5 x 3-15 µm.
India ink preparation:
Negative - no capsules present.
Dalmau plate culture:
Pseudohyphae and true hyphae are produced.
Molecular identification:
ITS sequencing recommended.
MALDI-TOF MS:
Able to accurately identify this species.
| Physiological Tests: + Positive, - Negative, v Variable, w Weak, s Slow, nd No Data | |||||||
|---|---|---|---|---|---|---|---|
| Germ Tube | - | L-Sorbose | v | L-Arabinose | - | D-Glucitol | + |
| Fermentation | Sucrose | - | D-Arabinose | - | 𝝰-M-D-Glucoside | - | |
| Glucose | - | Maltose | - | D-Ribose | v | D-Gluconate | v |
| Galactose | - | Cellobiose | w,- | L-Rhamnose | - | DL-Lactate | + |
| Sucrose | - | Trehalose | - | D-Glucosamime | - | myo-Inositol | - |
| Maltose | - | Lactose | - | N-A-D-glucosamine | + | 2-K-D-Gluconate | - |
| Lactose | - | Melibiose | - | Glycerol | + | D-Glucuronate | nd |
| Trehalose | - | Raffinose | - | Erythritol | + | Nitrate | - |
| Assimilation | Melezitose | - | Ribitol | v | Urease | - | |
| Glucose | + | Soluble Starch | - | Galactitol | - | 0.1% Cycloheximide | - |
| Galactose | v | D-Xylose | - | D-Mannitol | + | Growth at 37C | v |
Key features:
Germ tube negative yeast and sugar assimilation pattern.
| Antifungal | No | ≤0.03 | 0.06 | 0.125 | 0.25 | 0.5 | 1 | 2 | 4 | 8 | 16 | 32 | ≥64 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AMB | 26 | 3 | 1 | 1 | 6 | 7 | 6 | 1 | 1 | ||||
| FLU | 26 | 1 | 1 | 14 | 8 | 1 | 1 | ||||||
| VORI | 26 | 10 | 9 | 6 | 1 | ||||||||
| POSA | 26 | 1 | 4 | 5 | 11 | 4 | 1 | ||||||
| ITRA | 10 | 1 | 1 | 5 | 3 | ||||||||
| ANID | 26 | 6 | 3 | 3 | 5 | 4 | 3 | 2 | |||||
| MICA | 20 | 6 | 7 | 5 | 2 | ||||||||
| 5FC | 10 | 5 | 1 | 1 | 2 | 1 |