Bird Seed Agar
For the selective isolation of Cryptococcus neoformans and C. gattii.
Guizotia abyssinica (niger seed) 50 g
Glucose 1 g
KH2PO4 (potassium dihydrogen orthophosphate) 1 g
Creatinine 1 g
Bacto-agar (BD) 15 g
Distilled water 1000 mL
Additives: to each 500 ml bottle.
Penicillin G (20 units/ml) 1 mL
Gentamicin (40 mg/ml) 1 mL
Grind seeds of Guizotia abyssinica as finely as possible with an electric grinder and add to 1000 ml distilled water in a stainless steel jug.
Boil for 30 minutes, pass through filter paper and adjust volume to 1000 mL.
Add remaining ingredients except Bacto-agar to filtrate and dissolve.
If required: Cool to room temperature and pH to 5.5
Dispense into 500 mL bottles.
Add 7.5 g Bacto-agar to each 500 mL reagent bottle.
Autoclave at 121C for 20 minutes.
Cool to 48C and add 0.5 ml Penicillin G and 0.5 ml Gentamicin to each 500 ml of Bird Seed Agar.
Mix gently and pour into 90 mm plastic petri dishes.