Bromocresol Purple Milk Solids Glucose Agar (BCP).
For the differentiation of Trichophyton species (Fischer and Kane 1971; Summerbell et al. 1988).
Distilled water 1000 mL
Skim milk powder (Carnation brand) 80 g
Bromcresol (or bromocresol) purple (1.6% solution in alcohol) 2 mL
Dissolve in 2 litre flask and autoclave 121C for 10 minutes.
Glucose 40 g
Distilled water 200 mL
Dissolve and autoclave at 121C for 10 minutes.
Bacto agar (BD 214010) 30 g
Distilled water 800 mL
Soak for 15 minutes in 3 litre flask; autoclave at 121C for 10 minutes.
To make media add solution A and B to solution C. Adjust final pH to 6.6.
Aseptically dispense for slopes (7 mL amounts into bottles).