Cellotape Flag Preparations
An excellent technique for the rapid mounting of sporulating fungi because it keeps more of the reproductive structures intact.
Cellotape Flag Preparation.
Method
Using clear 2cm wide cellotape and a wooden applicator stick (orange stick) make a small cellotape flag (2x2 cm).
Using sterile technique, gently press the sticky side of the flag onto the surface of the culture.
Remove and apply a drop of 95% alcohol to the flag, this acts as a wetting agent and also dissolves the adhesive glue holding the flag to the applicator stick.
Place the flag onto a small drop of Lactophenol cotton blue on a clean glass slide, remove the applicator stick and discard, add another drop of stain, cover with a coverslip, gently press and mop up any excess stain.