Cornmeal Glucose Sucrose Yeast Extract Agar.

To stimulate sporulation in some zygomycetes, especially Saksenaea and Apophysomyces.

Ingredients

Cornmeal agar (BD) 17 g

Dextrose (glucose) 2 g

Sucrose 3 g

Yeast extract (Difco) 1 g

Distilled water 1000 ml

Method

Mix dry ingredients into 100 ml H₂O, boil remaining water.

Add boiling water to mixture and bring to boil.

Dispense for slopes.

Autoclave for 10 minutes at 121C, remove and slope.