Vitamin Free Agar (Trichophyton Agar No 1)
For the differentiation of Trichophyton species
Trichophyton agar No. 1 (BD) 11.8 g
Distilled water 200 ml
Add agar to water in stainless steel jug, allow to soak.
Bring to boil to dissolve, stirring constantly.
Once boiled remove immediately to avoid discolouration.
Dispense for slopes.
Autoclave at 121C for 10 minutes, remove and slope