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Unknown 29 = Aspergillus nidulans
Direct microscopy: Grocott’s methenamine silver (GMS) stained tissue section of lung showing dichotomously branched, septate hyphae typical of Aspergillus species.
Culture: Aspergillus nidulans complex on Czapek Dox agar showing typical plain green colony with dark red-brown cleistothecia developing within and upon the conidial layer. Reverse may be olive to drab-grey or purple-brown.
Microscopy: Conidial heads of Aspergillus nidulans complex are short columnar and biseriate. Conidiophores are usually short, brownish and smooth-walled. Conidia are globose and rough-walled.
Cleistothecium of Emericella nidulans (anamorph A. nidulans) showing numerous reddish-brown ascospores.
Cleistothecia of A. nidulans are often surrounded by a mass of Hülle cells which are up to 25 um in diameter.
Comment: Aspergillus nidulans complex is a typical soil fungus with a world-wide distribution. It has also been reported as a causative agent of aspergillosis in humans and animals.
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