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Unknown 52 = Lasiodiplodia theobromae
Culture: Colonies are greyish sepia to mouse grey to black, fluffy with abundant aerial mycelium; reverse fuscous black to black.
Growth on carnation leaf agar to promote pycnidia production. Pycnidia are simple or compound, often aggregated, stromatic, ostiolate, frequently setose, up to 5 mm wide.
Microscopy: Pycnidia are simple or compound, often aggregated, stromatic, ostiolate, frequently setose, up to 5 mm wide. Conidiophores are hyaline, simple, sometimes septate, rarely branched cylindrical, arising from the inner layers of cells lining the pycnidial cavity. Conidiogenous cells are hyaline, simple, cylindrical to subobpyriform, holoblastic, annellidic.
Conidia are initially unicellular, hyaline, granulose, subovoid to ellipsoid-oblong, thick-walled, base truncate; mature conidia one-septate, cinnamon to fawn, often longitudinally striate, 20-30 x 10-15 µm.
Key features: Coelomycete, with pycnidia producing characteristic two-celled, dark brown, striated conidia.
Comment: Lasiodiplodia theobromae is a well known plant pathogen and it has been reported from about 500 host plants, mainly confined to an area 40 degrees north to 40 degrees south of the equator. It has also been associated with mycotic keratitis, lesions on nail and subcutaneous tissue. RG-1 organism.
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