Candida metapsilosis
Candida metapsilosis is recognised as part of the Candida parapsilosis species complex (Tavanti et al., 2005).
It is phenotypically indistinguishable from C. parapsilosis, C. orthopsilosis and Lodderomyces elongisporusand is best identified by ITS sequencing or MALDI-ToF MS.
RG-2 organism.
Culture:
Colonies (SDA) white to cream-coloured smooth, glabrous, yeast-like.
Microscopy:
Ellipsoid, subglobose to fusiform budding blastoconidia, 4 x 3-6 µm, with some larger subglobose forms present.
India ink preparation:
Negative - no capsules present.
Dalmau plate culture:
Abundant, much branched pseudohyphae produced.
| Physiological Tests: + Positive, - Negative, v Variable, w Weak, s Slow, nd No Data | |||||||
|---|---|---|---|---|---|---|---|
| Germ Tube | - | L-Sorbose | + | L-Arabinose | + | D-Glucitol | + |
| Fermentation | Sucrose | + | D-Arabinose | - | 𝝰-M-D-Glucoside | + | |
| Glucose | + | Maltose | + | D-Ribose | + | D-Gluconate | + |
| Galactose | + | Cellobiose | - | L-Rhamnose | - | DL-Lactate | - |
| Sucrose | + | Trehalose | + | D-Glucosamime | - | myo-Inositol | - |
| Maltose | - | Lactose | - | N-A-D-glucosamine | + | 2-K-D-Gluconate | + |
| Lactose | - | Melibiose | - | Glycerol | + | D-Glucuronate | - |
| Trehalose | - | Raffinose | - | Erythritol | - | Nitrate | - |
| Assimilation | Melezitose | + | Ribitol | + | Urease | - | |
| Glucose | + | Soluble Starch | v | Galactitol | - | 0.1% Cycloheximide | - |
| Galactose | + | D-Xylose | + | D-Mannitol | + | Growth at 37C | + |
Key features:
Candida metapsilosis cannot be distinguished morphologically from C. parapsilosis and C. orthopsilosis, but can be identified by ITS sequencing (Asadzadeh et al. 2009, Borman et al. 2009, Tavanti et al. 2005) and MALDI-TOF MS analysis.
| Antifungal | No | ≤0.016 | 0.03 | 0.06 | 0.125 | 0.25 | 0.5 | 1 | 2 | 4 | 8 | 16 | 32 | ≥64 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AMB | 58 | 2 | 9 | 21 | 22 | 3 | 1 | |||||||
| FLU | 58 | 1 | 4 | 17 | 22 | 4 | ||||||||
| ISAV | 7 | 5 | 1 | 1 | ||||||||||
| VORI | 58 | 13 | 31 | 10 | 4 | |||||||||
| POSA | 58 | 6 | 3 | 15 | 27 | 5 | 1 | 1 | ||||||
| ITRA | 28 | 2 | 3 | 4 | 9 | 9 | 1 | |||||||
| ANID | 39 | 1 | 11 | 12 | 12 | 2 | 1 | |||||||
| MICA | 39 | 6 | 15 | 17 | 1 | |||||||||
| 5FC | 27 | 16 | 6 | 4 | 1 |
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