Candida metapsilosis

RG-2 organism.

Culture: 
Colonies (SDA) white to cream-coloured smooth, glabrous, yeast-like.

Microscopy: 
Ellipsoid, subglobose to fusiform budding blastoconidia, 4 x 3-6 µm, with some larger subglobose forms present.

India ink preparation: 
Negative - no capsules present.

Dalmau plate culture: 
Abundant, much branched pseudohyphae produced.

Physiological Tests: + Positive, - Negative, v Variable, w Weak, s Slow, nd No Data
Germ Tube - L-Sorbose + L-Arabinose + D-Glucitol +
Fermentation   Sucrose + D-Arabinose - 𝝰-M-D-Glucoside +
Glucose + Maltose + D-Ribose + D-Gluconate +
Galactose + Cellobiose - L-Rhamnose - DL-Lactate -
Sucrose + Trehalose + D-Glucosamime - myo-Inositol -
Maltose - Lactose - N-A-D-glucosamine + 2-K-D-Gluconate +
Lactose - Melibiose - Glycerol + D-Glucuronate -
Trehalose - Raffinose - Erythritol - Nitrate -
Assimilation   Melezitose + Ribitol + Urease -
Glucose + Soluble Starch v Galactitol - 0.1% Cycloheximide -
Galactose + D-Xylose + D-Mannitol + Growth at 37C +

Key features:
Candida metapsilosis cannot be distinguished morphologically from C. parapsilosis and C. orthopsilosis, but can be identified by ITS sequencing (Asadzadeh et al. 2009, Borman et al. 2009, Tavanti et al. 2005) and MALDI-TOF MS analysis.

Antifungal susceptibility: Candida metapsilosis (Australian national data); MIC µg/mL.
Antifungal No ≤0.008 0.016 0.03 0.06 0.125 0.25 0.5 1 2 4 8 16 32 ≥64
AMB 32         1 5 12 11 2 1        
FLU 32           1   19 10 2        
ISAV 2 2                          
VORI 32 1 4 22 3 2                  
POSA 32   2 7 16 6 1                
ITRA 2         1 1                
ANID 13         2 5 3 2 1          
MICA 13           9 3 1            
CAS 26     1 5 15 4   1            
5FC 2       2                    

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