Confocal Microscopes

A1R

Nikon A1R Laser Scanning Confocal with DS-Ri1 CCD camera

Please contact Dr Gwen Mayo for further information.
Located at Waite facility.

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  • Information on the Nikon A1R Laser Scanning Confocal with DS-Ri1 CCD camera

    The Nikon A1R laser scanning confocal is a research grade confocal microscope capable of high resolution imaging.

    It has three solid state lasers (405nm, 561nm and 640nm) and a multi-line Argon gas laser (457nm, 488nm, 514nm).

    The unique hybrid scan head has a galvano scanner for high resolution imaging and a resonant scanner for high speed acquisition. The resonant scanner allows high-speed imaging ranging from 30 fps (512 x 512 pixels) to 420 fps (512 x 32 pixels), and the galvano scanner features a maximum scan rate of 4 frames per second at 512 x 512 pixels. By employing a hexagonal pinhole, higher brightness than that of a circular pinhole is achieved.

    The A1R confocal has two detectors. The “standard” 4 channel hybrid detector has two GaAsP PMTs and two normal PMTs. The spectral detector has wavelength resolutions of 2.5, 6, and 10 nanometers, and a 32-channel multi-anode photomultiplier. Spectral unmixing allows separation of closely overlapping fluorescence spectra and the elimination of autofluorescence. Real time unmixing can be applied during image acquisition.

    The Ni-E motorized microscope is sited on TMC vibration isolation table. The microscope has two dry objectives (10X Plan Apochromat Lambda; 20X Plan Apochromat Lambda), two water immersion objectives (40X Apo LWD WI λS; 60x Plan Apo VC WI) and one oil immersion objective (100X Plan Apochromat Lambda H Oil, N.A. 1.45, W.D.0.13mm). Fluorescence filters are longpass: V-2A (Ex 330-380, DM 400 BA 420); UV-2A (Ex 380-420, DM 430 BA 450); B-2A (Ex 450-490, DM 505 BA 520); G-2A (Ex 510-560, DM 575 BA 590).

    The microscope has a zooming port and DS-Ri1 colour cooled digital camera for widefield (brightfield and fluorescence) imaging. An additional GFP band-pass filter is available for widefield imaging. The NIS-Elements C control software platform incorporates visualization, image analysis, processing and data management.

    For more information on the manufacturer visit Nikon web site.

Olympus FV3000 2

Olympus FV3000 Confocal Microscope

Please contact Dr Agatha Labrinidis (AHMS) or Dr Jane Sibbons (MLS) for further information.
Located at Adelaide Health & Medical Sciences Building and Molecular Life Sciences Building.  

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  • Information on the Olympus FV3000 Confocal Microscope

    The Olympus FV3000 Laser Scanning Microscope is a fully spectral microscope with a brand new TruSpectral Technology that combines high performance imaging capabilities with customisable software that is easy to use. Main features include: Fast and accurate imaging using a high-speed scanners, bright fluorescent imaging of even dim samples due to high-sensitivity detectors and improved observation reliability achieved by precise spectroscopy and newly developed time management system.

    APPLICATIONS
    The FLUOVIEW FV3000 series is designed to meet some of the most difficult challenges in modern science. Featuring the high sensitivity and speed required for live cell and tissue imaging, the FV3000 enables 2D-6D (x,y,z,t,λ,p) macro-to-micro imaging of cells, tissues, and small organisms. With an intuitive and adaptable user interface, the FV3000 supports complete workflows from image acquisition to processing and analysis. Particular attention has been paid to the needs of cell biology, cancer research, and stem cell research, and with two new upright configurations, the FV3000 is also poised to meet the needs of neuroscience, electrophysiology, and developmental biology.

    FEATURES

    • The FV3000 enables macro scale imaging from 1.25X while retaining capabilities of super resolution imaging at up to 150X magnification.
    • Silicon immersion objectives for accurate 3D imaging.
    • Resonant scanner to capture highly dynamic biological processes.
    • Multi-Dimensional Time-lapse with Z-drift compensator.
    • 3D Mosaic Imaging

    CAPABILITIES
    A wide range of live and post processing analysis functions are available to enhance data production:

    • Fluorescence spectral unmixing (up to 16 channels)
    • 2D data histogram display
    • Ratio imaging and IMD display
    • Fluorescence intensity and time-lapse measurements
    • Rolling average processing
    • Projections (including maximum intensity Z)
    • Z gap correction
    • Image editing (including individual color setting, pseudo-color, and graphic/text annotation)
    • FRET
    • FRAP
    • Super-Resolution
    • Noise Reduction

    Featured cellSens Image Processing and Analysis Functions

    • Constrained Iterative Deconvolution
    • Count and Measure (Object Segmentation, Measurement, Counting, Classification)
    • Kymography (Velocity Measurements)
    • One-click macros for automated processing and analysis
Profilometer

Olympus LEXT OLS5000 Profilometer

Please contact Dr Benjamin Wade for further information.
Located at Frome Road.

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  • Information on the Olympus LEXT OLS5000 Profilometer

    The Olympus LEXT OLS5000 is a laser confocal microscope designed specifically to measure shape and surface roughness of samples at the submicron level. 

    It is a complementary tool to that of AFM which is traditionally and predominantly used for high resolution height and surface roughness measurements of much smaller areas.  The instrument utilises a 405nm violet laser and a pinhole aperture much the same as traditional confocal microscopes, with the addition of a MEMS resonant scanner used for XY scanning of the UV laser for low distortion and aberrations.

    When measuring height of the sample, the microscope acquires multiple confocal images through a Z range defined by the user.  Based on focus position (Z) and detected light intensity, light intensity variation curves are created for each pixel.  Following this a height information/image can be reconstructed, along with an “extended” image which is an image of the sample surface in which the entire surface is in focus.

    • Sample sizes up to 100mm high
    • 100mm x 100mm motorised stage
    •  5x, 10x, 20x, 50x LEXT normal WD objectives, 20x, 100x long WD objectives
    • DIC option
    • 4k x 4k resolution of scanned images
    • CMOS colour camera for optical image
    • Height/Width Accuracy: measurement value +/- 1.5%
    • For full range of specifications click here

    Samples:

    • Providing sample can fit onto stage and doesn’t exceed weight limit (2-3kg), the full range of samples including but not limited to biological, metallurgical, and geological can be analysed.

    Height map of laser ablation pit in silicate glass
     

    Volume measurement of laser ablation pit using Olympus LEXT software
     

    Scratch depth measurements in glass using Olympus LEXT software
     

    Surface roughness measurements of glass sample using Olympus LEXT software