PLANT SC 7226WT - Molecular Plant Breeding
Waite Campus - Winter - 2014
General Course Information
Course Code PLANT SC 7226WT Course Molecular Plant Breeding Coordinating Unit School of Agriculture, Food and Wine Term Winter Level Postgraduate Coursework Location/s Waite Campus Units 3 Contact Up to 24 hours per week for 3 weeks Assumed Knowledge PLANT SC 7225WT Restrictions Available to GradCertPHB, GradDipPHB, MHB students only Course Description This course involves teaching sessions that may be attended by both Undergraduate and Postgraduate students.
Plant molecular biology can be incorporated into crop improvement programs via plant transformation (gene technology) and/or via the application of genetic marker information. Plant cell and tissue culture is used in plant transformation and has other applications in plant breeding. This course considers the scientific basis for the application of plant transformation, molecular markers and cell and tissue culture in plant breeding.
Course Coordinator: Professor Diane Mather
The full timetable of all activities for this course can be accessed from Course Planner.
Course Learning OutcomesStudents will be able to:
- Understand and explain scientific principles behind plant cell and tissue culture, plant transformation, molecular markers and genome mapping.
- Analyse information from plant molecular biology research and recognize its potential applications in crop improvement.
- Synthesize information from plant molecular biology and plant breeding to design plant molecular breeding strategies.
- Evaluate the relative merits of plant transformation, marker-assisted breeding and conventional phenotypic selection for particular situations.
- Demonstrate skills in collaborative group learning processes, emergent technologies and the ability to apply these principles to a specified project.
University Graduate Attributes
University Graduate Attribute Course Learning Outcome(s) Knowledge and understanding of the content and techniques of a chosen discipline at advanced levels that are internationally recognised. 1 The ability to locate, analyse, evaluate and synthesise information from a wide variety of sources in a planned and timely manner. 2,3,4 An ability to apply effective, creative and innovative solutions, both independently and cooperatively, to current and future problems. 3,4,5 Skills of a high order in interpersonal understanding, teamwork and communication. 5 A proficiency in the appropriate use of contemporary technologies. 5 A commitment to continuous learning and the capacity to maintain intellectual curiosity throughout life. 1 A commitment to the highest standards of professional endeavour and the ability to take a leadership role in the community. 1-5 An awareness of ethical, social and cultural issues within a global context and their importance in the exercise of professional skills and responsibilities. 1-5
Required ResourcesLab coats and closed-in shoes are required for laboratory work.
Recommended ResourcesIt is recommended that students read sections 20.1-20.5, 20.11 and 20.12 and Chapter 22 of the following book:
- Acquaah, G. 2012, Principles of Plant Genetics and Breeding, 2nd edition, Blackwell Publishing, Malden.
- Xu, Y. 2010 'Gene transfer and genetically modified plants' Pp 458-500 In Xu, Y. (Ed.) Molecular Plant Breeding, CABI.
- Garcia, M & Mather DE 2014 ‘From genes to markers: exploiting gene sequence information to develop tools for plant breeding’, In Fleury, D & Whitford, R (eds.) Crop Breeding: Methods and Protocols. Methods in Molecular Biology vol. 1145, Springer Science+Business Media, New York.
Online LearningTeaching materials and course documentation will be posted on the MyUni website. MyUni will be used extensively by academic staff and students .
Learning & Teaching Activities
Learning & Teaching ModesThe course includes a series of lectures, complemented by practical sessions and problem-based group learning sessions.
The information below is provided as a guide to assist students in engaging appropriately with the course requirements.A full-time student in a 3-unit course should expect to spend a total of 156 hours on their studies. This includes both the formal contact time required in the course (e.g. lectures, group work, practicals), as well as non-contact time (e.g. reading, writing and revision).
Learning Activities Summary
Schedule Date Day Start End Activity Instructor June 16 Monday 9 10 Introduction to molecular plant breeding Diane Mather 10 11 From genomics to molecular plant breeding - examples from rice Sigrid Heuer 2 4 Group work Chad Habel, Diane Mather 17 Tuesday 9 11 GM breeding: achievements and approaches Diane Mather 18 Wednesday 9 1 LAB PRAC: Agrobacterium-mediated transformation (Waite GS) Nannan Yang 2 4 Group work Chad Habel 19 Thursday 9 11 GM breeding: regulatory and commercial considerations Diane Mather 11 12 Visit to PC facility (containment glasshouse) Jan Nield 20 Friday 9 1 LAB PRAC: Biolistic transformation (Waite GS) Nannan Yang 3 5 Group work Chad Habel 23 Monday 9 11 Novel plant breeding technologies Diane Mather 11 12 Transformation methods Q&A Nannan Yang, Rohan Singh 3 5 Group work Chad Habel, Diane Mather 24 Tuesday 9 11 Markers in plant breeding 1 Diane Mather 1 3 COMPUTER PRAC: From genes to markers (CH 129) Melissa Garcia 25 Wednesday 9 1 LAB PRAC: Marker-assisted breeding - part 1 Tim March 2 4 Group work Chad Habel 26 Thursday 9 11 Markers in plant breeding 2 Diane Mather 1 2 Molecular methods in hybrid breeding Ryan Whitford 27 Friday 9 1 LAB PRAC: Marker-assisted breeding part 2 Tim March 30 Monday 9 10 Genomic selection Diane Mather 10 11 Next-generation mutation breeding: TILLING and EcoTILLING Diane Mather 2 4 Group work Chad Habel, Diane Mather July 1 Tuesday 9 10 Application of molecular methods in commercial wheat breeding Haydn Kuchel 10 11 Application of molecular methods in almond breeding Michelle Wirthensohn 2 Wednesday 9 1 COMPUTER PRAC: Understanding marker data (CH 129) Ken Chalmers 2 4 Group work Chad Habel 3 Thursday 9 10 Applications of tissue culture in plant breeding Diane Mather 10 11 From transgenic plant to transgenic cultivar: the Golden Rice experience Diane Mather 4 Friday 11 1 Review session Diane Mather 2 4 Group work Chad Habel
Small Group Discovery ExperienceThe group problem-based learning project is a small group discovery experience.
- Assessment must encourage and reinforce learning.
- Assessment must enable robust and fair judgements about student performance.
- Assessment must maintain academic standards.
Assessment Task Task Type Due Weighting Learning Outcome Group learning project Summative
Friday 11 July
35% LO2 - LO5 Short assignments (on-line questions posted on MyUni) Summative To be scheduled during the course 30% LO1 Final examination Summative Tuesday 8 July 35% LO1 - LO4
Short assignmentsDuring the course, students will be expected to answer short questions related to the course content. Questions and due dates will be posted on MyUni. One purpose of these assignments is to encourage students to keep up with the reading and lecture materials.
Group projectStudents are assigned to work in groups, as consultants to the director of a research centre. Each group will prepare a report (up to 3000 words) on whether and how the CRISPR/Cas9 system could be used to address an assigned plant breeding objective. Each report should include:
- An executive summary
- An introduction to the plant breeding objective
- An introduction to CRISPR/Cas9 technology and the opportunity it presents to address the objective
- A specific plan for what would need to be done in order to successfully used CRISPR/Cas9 to address the objective
- A discussion of the key considerations that the client will need to take into account in order to decide whether to proceed with this as pilot project. This discussion does not need to be limited to scientific factors, but could also take regulatory (biosafety) and/or commercial factors into account. This discussion should give some consideration to whether there are alternative ways to address the objective and whether CRISPR/Cas9 has advantages or disadvantages relative to these alternatives.
- A list of recommendations that will be helpful to the client.
- A list of references cited in the report.
- The extent to which the report demonstrates a thorough understanding of the assigned plant breeding objective and the CRISPR/Cas9 system (5 marks)
- The scientific validity and feasibility of the plan (10 marks)
- Professional presentation and clarity of the report (10 marks)
- Whether the recommendations are logical and consistent with the information presented (5 marks)
- Whether the plan uses references appropriately and thoroughly (5 marks)
- Moderation based on any observed inequality of contributions by group members, based on observations of instructors in combination with student input in an anonymous peer assessment survey (± between 0 and 5 marks).
Final examinationThe final examination will be a three-hour written examination, with questions designed to assess your understanding of the concepts covered in the course.
SubmissionAll written work is to be submitted via MyUni.
Grades for your performance in this course will be awarded in accordance with the following scheme:
M10 (Coursework Mark Scheme) Grade Mark Description FNS Fail No Submission F 1-49 Fail P 50-64 Pass C 65-74 Credit D 75-84 Distinction HD 85-100 High Distinction CN Continuing NFE No Formal Examination RP Result Pending
Further details of the grades/results can be obtained from Examinations.
Final results for this course will be made available through Access Adelaide.
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