PLANT SC 7226WT - Molecular Plant Breeding
Waite Campus - Winter - 2015
General Course Information
Course Code PLANT SC 7226WT Course Molecular Plant Breeding Coordinating Unit School of Agriculture, Food and Wine Term Winter Level Postgraduate Coursework Location/s Waite Campus Units 3 Contact Up to 24 hours per week for 3 weeks Available for Study Abroad and Exchange Y Assumed Knowledge PLANT SC 7225WT Restrictions Available to GradCertPHB, GradDipPHB, MHB students only Course Description This course involves teaching sessions that may be attended by both Undergraduate and Postgraduate students.
Plant molecular biology can be incorporated into crop improvement programs via plant transformation (gene technology) and/or via the application of genetic marker information. Plant cell and tissue culture is used in plant transformation and has other applications in plant breeding. This course considers the scientific basis for the application of plant transformation, molecular markers and cell and tissue culture in plant breeding.
Course Coordinator: Professor Diane Mather
The full timetable of all activities for this course can be accessed from Course Planner.Please see the Learning & Teaching Activities section of this course outline for a detailed schedule of learning activities for 2015.
Course Learning OutcomesStudents will be able to:
- Understand and explain scientific principles behind plant cell and tissue culture, plant transformation, molecular markers and genome mapping.
- Analyse information from plant molecular biology research and recognize its potential applications in crop improvement.
- Synthesize information from plant molecular biology and plant breeding to design plant molecular breeding strategies.
- Evaluate the relative merits of plant transformation, marker-assisted breeding and conventional phenotypic selection for particular situations.
- Demonstrate skills in collaborative group learning processes, emergent technologies and the ability to apply these principles to a specified project.
University Graduate Attributes
This course will provide students with an opportunity to develop the Graduate Attribute(s) specified below:
University Graduate Attribute Course Learning Outcome(s) Knowledge and understanding of the content and techniques of a chosen discipline at advanced levels that are internationally recognised. 1 The ability to locate, analyse, evaluate and synthesise information from a wide variety of sources in a planned and timely manner. 2,3,4 An ability to apply effective, creative and innovative solutions, both independently and cooperatively, to current and future problems. 3,4,5 Skills of a high order in interpersonal understanding, teamwork and communication. 5 A proficiency in the appropriate use of contemporary technologies. 5 A commitment to continuous learning and the capacity to maintain intellectual curiosity throughout life. 1 A commitment to the highest standards of professional endeavour and the ability to take a leadership role in the community. 1-5 An awareness of ethical, social and cultural issues within a global context and their importance in the exercise of professional skills and responsibilities. 1-5
Required ResourcesLab coats and closed-in shoes are required for laboratory work.
Prior to the course, students should read
- Tester, M & Langridge P 2010 ‘Breeding technologies to increase crop production in a changing world’ Science 327: 818-822. This article provides a short introduction to a range of molecular breeding technologies.
- Garcia, M & Mather DE 2014 ‘From genes to markers: exploiting gene sequence information to develop tools for plant breeding’, In Fleury, D & Whitford, R (eds.) Crop Breeding: Methods and Protocols. Methods in Molecular Biology vol. 1145, Springer Science+Business Media, New York. This chapter provides essential background that you will need for the computer practical session that you will do on the first day of the course.
Recommended ResourcesFor additional background on molecular markers and marker-assisted selection, students may wish to read sections 20.1-20.5, 20.11 and 20.12 and Chapter 22 of the following book:
- Acquaah, G. 2012, Principles of Plant Genetics and Breeding, 2nd edition, Blackwell Publishing, Malden.
- Xu, Y. 2010 'Gene transfer and genetically modified plants' Pp 458-500 In Xu, Y. (Ed.) Molecular Plant Breeding, CABI.
Online LearningTeaching materials and course documentation will be posted on the MyUni website. MyUni will be used extensively by academic staff and students .
Learning & Teaching Activities
Learning & Teaching ModesThe course includes a series of lectures, complemented by practical sessions and problem-based group learning sessions.
The information below is provided as a guide to assist students in engaging appropriately with the course requirements.A full-time student in a 3-unit course should expect to spend a total of 156 hours on their studies. This includes both the formal contact time required in the course (e.g. lectures, group work, practicals), as well as non-contact time (e.g. reading, writing and revision).
Learning Activities Summary
Schedule Date Day Start End Activity Instructor June 15 Monday 9 11 LECTURES
Introduction to the course
Markers in plant breeding
Diane Mather 1 3 COMPUTER PRAC
From genes to markers
(CH Computer Suite 2)
Melissa Garcia 16 Tuesday 9 11 LECTURES
Markers in plant breeding (continued)
Diane Mather 1 4 GROUP WORK Jessica Scott
17 Wednesday 9 1 LAB PRAC
Marker-assisted breeding - part 1
Tim March 18 Thursday 11 12 LECTURE
Diane Mather 1 4 GROUP WORK Jessica Scott
19 Friday 9 1 LAB PRAC
Marker-assisted breeding - part 2
Tim March 22 Monday 9 11 LECTURE
GM breeding: achievements and approaches
Diane Mather 23 Tuesday 9 11 LECTURE
GM breeding; regulatort and commercial considerations
Diane Mather 1 4 GROUP WORK Jessica Scott 24 Wednesday 9 1 LAB PRAC
Transformation - part 1
Darren Plett and staff 2 3 VISIT
Containment facility: PC2 glasshouse
Jan Nield 25 Thursday 1 12 LECTURE
From transgenic plant to transgenic cultivar: the Golden Rice experience
Diane Mather 1 4 GROUP WORK Jessica Scott 26 Friday 9 1 LAB PRAC:
Transformation - part 2
Darren Plett and staff 29 Monday 9 11 LECTURES
Applications of tissue culture in plant breeding
Next-generation mutation breeding: TILLING and eco-TILLING
Diane Mather 30 Tuesday 9 1 LECTURES
Novel plant breeding technologies
Diane Mather 1 4 GROUP WORK Jessica Scott July 1 Wednesday 9 10 LECTURE
Application of biotechnology in commercial wheat breeding
Haydn Kuchel 10:30 11:30 LECTURE
Application of biotechnology in almond breeding
Michelle Wirthensohn 12 1 LECTURE
Application of biotechnology in ornamental eucalypt breeding
Kate Delaporte 2 Thursday 11 12 LECTURE
Biotechnology in hybrid breeding
Ryan Whitford 1 4 GROUP WORK Jessica Scott 3 Friday 11 1 Review session Diane Mather
Small Group Discovery ExperienceThe group problem-based learning project is a small group discovery experience.
The University's policy on Assessment for Coursework Programs is based on the following four principles:
- Assessment must encourage and reinforce learning.
- Assessment must enable robust and fair judgements about student performance.
- Assessment must maintain academic standards.
Assessment Task Task Type Due Weighting Learning Outcome Group learning project Summative
Friday 10 July
35% LO2 - LO5 Short assignments (on-line questions posted on MyUni) Summative To be scheduled during the course 30% LO1 Final examination Summative Tuesday 7 July 35% LO1 - LO4
Short assignmentsDuring the course, students will be expected to answer short questions related to the course content. Questions and due dates will be posted on MyUni. One purpose of these assignments is to encourage students to keep up with the reading and lecture materials.
Group projectStudents are assigned to work in groups, as consultants to a breeder of durum wheat. Each group will prepare a report (up to 3000 words) on whether and how a specific approach could be used to introduce the lutein esterification trait into durum wheat. Each report should include:
- An executive summary
- A brief introduction to the breeding objective
- An introduction to the assigned approach
- A realistic plan for how the assigned approach could be applied to this problem
- A discussion of the advantages, disadvantages and risks associated with the assigned approach
- A list of recommendations that will be helpful to your client.
- A list of references cited in the report
- The extent to which it demonstrates a thorough understanding of the problem and the assigned approach (10 marks)
- The scientific validity and feasibility of the recommended plan (10 marks)
- Professional presentation and clarity of the report (5 marks)
- Whether the recommendations are logical and consistent with the information presented (5 marks)
- Whether the report uses references appropriately and thoroughly (5 marks)
Final examinationThe final examination will be a three-hour written examination, with questions designed to assess students' understanding of the concepts covered in the course.
SubmissionAll written work is to be submitted via MyUni.
Grades for your performance in this course will be awarded in accordance with the following scheme:
M10 (Coursework Mark Scheme) Grade Mark Description FNS Fail No Submission F 1-49 Fail P 50-64 Pass C 65-74 Credit D 75-84 Distinction HD 85-100 High Distinction CN Continuing NFE No Formal Examination RP Result Pending
Further details of the grades/results can be obtained from Examinations.
Grade Descriptors are available which provide a general guide to the standard of work that is expected at each grade level. More information at Assessment for Coursework Programs.
Final results for this course will be made available through Access Adelaide.
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