MICRO 2504 - Microbiology II (Biotechnology)
North Terrace Campus - Semester 1 - 2015
General Course Information
Course Code MICRO 2504 Course Microbiology II (Biotechnology) Coordinating Unit School of Biological Sciences Term Semester 1 Level Undergraduate Location/s North Terrace Campus Units 3 Contact Up to 5 hours per week Available for Study Abroad and Exchange N Prerequisites BIOLOGY 1101 & BIOLOGY 1201 or BIOLOGY 1202 Incompatible MICRO 2500, MICRO 2502, MICRO 2000, MICRO 2004 & MICRO 3003 Assumed Knowledge CHEM 1100 or CHEM 1101 & CHEM 1200 or CHEM 1201 Restrictions Available to B Sc (Biotechnology) students only Course Description This course is an introduction to microbiology that provides a strong grounding in fundamental aspects of the basic biology of bacteria and bacterial viruses as well as aspects of molecular biology and genetics. Emphasis is placed on biotechnological applications of bacteria such as the cloning of bacterial genes, expression of recombinant proteins for therapeutic and industrial uses and development of biological control agents. Topics covered include: introduction to microorganisms and their environment, microbial structure and function; microbial molecular biology and genetics; bacterial viruses; new and emerging pathogens of humans and other animals; mechanisms by which micro-organisms cause disease in plants and animals; biotechnological applications of bacteria. Students enrolled in this course will attend one or more of Practicals A, B and C offered by the School of Molecular and Biomedical Science. Refer to Current Students Online Enrolment information at www.sciences.adelaide.edu.au for further information.
Course Coordinator: Dr Connor Thomas
The full timetable of all activities for this course can be accessed from Course Planner.
Course Learning Outcomes
At the end of the course students should:
- Have an appreciation of the practice of microbiology.
- Be able to describe the structure of bacterial cells, the form, arrangement and replication of genetic material within a bacterial cell; the types of mutations that may occur in bacterial DNA, evolution of bacteria; the use of nucleic acid in the molecular taxonomy of bacterial species.
- Be familiar with the mechanisms by which bacteria can exchange fragments of DNA, the mechanisms by which self-transmissible DNA fragments maintain their presence in bacterial cells and how they may mediate their own transfer to other bacterial cells.
- Be able to describe the replication strategies used by bacterial viruses.
- Be able to describe the nutritional and physical requirements for bacterial growth and explain the dynamics of the growth of a bacterial population and how this growth can be measured.
- Be able to describe the principles involved in killing bacteria, and make recoomendations on use of physical and chemical methods used to control microbial growth, including how the effectiveness of heat treatments is measured.
- Have an understanding of how proteins are secreted and exported to different cellular compartments and appreciate the role of membrane and cell wall associated proteins in nutrient transport and microbial pathogenesis.
- Be familiar with the ways in which bacterial pathogens can be transmitted to humans, and the factors that influence transmission of pathogens and the occurrence of infectious diseases. This includes the concepts of virulence and virulence factors, opportunistic pathogens, and predisposing factors to disease.
- Appreciate the barriers that are used by the host to resist bacterial pathogens, the mechanisms whereby innate (natural) and adaptive (acquired) immunity provide protection against infectious agents, and the role of vaccines in protection of animal hosts from infection.
- Understand how the plant pathogen, Agrobacterium tumefaciens, can be used to construct transgenic plants.
University Graduate Attributes
This course will provide students with an opportunity to develop the Graduate Attribute(s) specified below:
University Graduate Attribute Course Learning Outcome(s) Knowledge and understanding of the content and techniques of a chosen discipline at advanced levels that are internationally recognised. 1 - 10 The ability to locate, analyse, evaluate and synthesise information from a wide variety of sources in a planned and timely manner. 2, 3, 7, 8, 10 An ability to apply effective, creative and innovative solutions, both independently and cooperatively, to current and future problems. 3, 4, 6, 7, 10 Skills of a high order in interpersonal understanding, teamwork and communication. 1, 5, 6, 10 A proficiency in the appropriate use of contemporary technologies. 2, 3, 6, 9, 10 A commitment to continuous learning and the capacity to maintain intellectual curiosity throughout life. 1 A commitment to the highest standards of professional endeavour and the ability to take a leadership role in the community. 1 An awareness of ethical, social and cultural issues within a global context and their importance in the exercise of professional skills and responsibilities. 1
Required ResourcesJ.M. Willey, L.M. Sherwood and C.J. Woolverton. Prescott's Microbiology. 9th Edition. McGraw Hill.
Online LearningTeaching materials and course documentation will be posted on the MyUni website (http://myuni.adelaide.edu.au/).
Learning & Teaching Activities
Learning & Teaching ModesThis course will be delivered in the following means:
- 24 x 1 hour lectures and 8 x 1 hour tutorials delivered over 12 weeks.
- Practical sessions as required. Practical class contact of 4hr per week for up to five weeks during semester, as required according to the MBS Practical ABC system (see Course Planner for details, under SCIENCE 2100/2101/2102 as required).
The information below is provided as a guide to assist students in engaging appropriately with the course requirements.
A student enrolled in a 3 unit course, such as this, should expect to spend, on average 12 hours per week on the studies required. This includes both the formal contact time required to the course (e.g., lectures and practicals), as well as non-contact time (e.g., reading and revision).
Learning Activities SummaryPracticals for this course are Pracs ABC which involves additional enrolment
*Optional teaching week
Schedule Week Type of learning activity Topic Week 1 Lecture Introduction to the Microbial World
Morphology and Structure of Bacteria
Diversity of Bacteria
Practical Tutorial or other activity Week 2 Lecture Factors affecting Growth of Bacteria I
Factors affecting Growth of Bacteria II
Factors affecting Growth of Bacteria III
Practical Tutorial or other activity Review of bacterial structure and function Week 3 Lecture Cultivation and Identification of Bacteria
Cell Membranes and Membrane Associated Functions
Cell Wall Synthesis and Assembly
Practical Tutorial or other activity Growth of bacterial populations Week 4 Lecture Nutrient Uptake
Protein Secretion in Gram positive and negative bacteria
No scheduled lecture
Practical Tutorial or other activity Inactivation of bacteria. Week 5 Lecture No scheduled lecture
No scheduled lecture
Microbiology Test 1 (Weeks 1 to 4)
Practical Tutorial or other activity Week 6 Lecture Bacterial Genetics and Molecular Biology II
Bacterial Genetics and Molecular Biology I
Recombinant DNA Technology Fundamentals I
Practical Tutorial or other activity Protein secretion in bacteria Week 7 Lecture Recombinant DNA Technology Fundamentals II
Lateral Gene Transfer and Gene Knockouts
Jumping Genes: IS elements, Transposons &
Practical Tutorial or other activity Week 8 Lecture Bacteriophage Diversity & Interaction with Host Cells I
Bacteriophage Diversity & Interaction with Host Cells II
No scheduled lecture
Practical Tutorial or other activity Fundamentals of bacterial recombinant DNA technology. Week 9 Lecture No scheduled lecture
No scheduled lecture
Microbiology Test 2 (Weeks 6 to 8)
Practical Tutorial or other activity Week 10 Lecture Bacteria and Human Health
Colonization to Disease
Bacterial Survival in the Host
Practical Tutorial or other activity Lateral gene transfer. Week 11 Lecture Virulence factors; Mechanisms for Disease
No scheduled lecture
Agrobacterium: An Extraordinary Plant Pathogen
Practical Tutorial or other activity Microbial pathogenesis. Pathology of Urinary tract infections caused by Escherichia coli. Week 12 Lecture Biotechnology of Transgenic Plants
Water Treatment & Testing, Water-borne Pathogens
Practical Tutorial or other activity Bioloogy and molecular biology of plant infections by Agrobacterium tumefaciens. Week 13* Lecture Optional Theory Revision Session I
Optional Theory Revision Session II
Optional Theory Revision Session III
Practical Tutorial or other activity
The University's policy on Assessment for Coursework Programs is based on the following four principles:
- Assessment must encourage and reinforce learning.
- Assessment must enable robust and fair judgements about student performance.
- Assessment practices must be fair and equitable to students and give them the opportunity to demonstrate what they have learned.
- Assessment must maintain academic standards.
Assessment Task Task Type Hurdle Weighting Practical Assessment Summative
20% Tutorials Summative No 10% Theory tests Summative No 30% Final Exam Summative No 40%
Assessment Task Type of assessment Percentage
of total assessment for grading purposes
Practical Assessment Summative 20% No Tutorials Summative 10% No Theory tests Summative 30% No Final Exam Summative 40% No
The assessment methods used for this course are designed to test development of various Bachelor of Science graduate attributes. The components of assessment used are:
Practical assessment: 20%
This summative and formative activity will assist students to develop an understanding of the observational and experimental character of science, to value the close relationship between scientific research and development of new knowledge, develop practical laboratory skills, appreciate the need for experimental design, develop skills to interpret raw experimental data. The practical exercises are designed to develop teamwork, objective criticism and high level numerical and computing skills. The skills to effectively communicate the outcomes in written and oral reports is a significant part. The laboratory exercises will give students opportunity to learn skills made available by new technologies. The practicals include laboratory based exercises, computer based exercises to develop data handling skills, use of online databases and bioinformatics applications, and online journal and abstracting systems for preparation of review style reports. The practicals are assessed by a combination of written reports and oral presentations.
All tutorials are compulsory. The aim of tutorials is to develop a student’s knowledge, scientific curiosity for microbiology and an appreciation of the role of microbiology for society and the environment. The tutorials are designed to reinforce lectures, assist students to develop a deeper understanding of the theory, allow students to explore the use of experimental methods to test hypotheses. Assessment of tutorials is by a mix of multiple choice question sets, short answer questions and evaluation of student performance in discussion.
Theory Tests: 30%
This summative assessment activity will address the learning outcomes (objectives) for theory delivered in the first term of the semester. One test will be scheduled at the end of Term 1 and the other at mid-Term 2. Test formats will be published in advance and may comprise multiple choice question sets, questions requiring one or two sentence answers or short answer questions designed to test understanding and application of the theory component of the course are used. Feedback on test outcomes is used to assist students to address learning difficulties, to refine frameworks for communication of scientific information and problem solving.
End of Semester Exam: 40%
This summative assessment activity will comprehensively address the learning outcomes (objectives) of this course. This assessment uses a mix of multiple choice question sets, brief answer questions (requiring at most 1 or 2 sentences to answer), short answer questions (10 minute questions requiring answers of about 1 page of written material) to test student comprehension of the theory part of the course. In particular, the 10 minute short answer questions are used to test ability to apply the theory to solve practical problems, and development of logical thought within the framework of the scientific method.
If an extension is not applied for, or not granted then a penalty for late submission will apply. A penalty of 10% of the value of the assignment for each calendar day that is late (i.e. weekends count as 2 days), up to a maximum of 50% of the available marks will be applied. This means that an assignment that is 5 days or more late without an approved extension can only receive a maximum of 50% of the mark.
Grades for your performance in this course will be awarded in accordance with the following scheme:
M10 (Coursework Mark Scheme) Grade Mark Description FNS Fail No Submission F 1-49 Fail P 50-64 Pass C 65-74 Credit D 75-84 Distinction HD 85-100 High Distinction CN Continuing NFE No Formal Examination RP Result Pending
Further details of the grades/results can be obtained from Examinations.
Grade Descriptors are available which provide a general guide to the standard of work that is expected at each grade level. More information at Assessment for Coursework Programs.
Final results for this course will be made available through Access Adelaide.
The University places a high priority on approaches to learning and teaching that enhance the student experience. Feedback is sought from students in a variety of ways including on-going engagement with staff, the use of online discussion boards and the use of Student Experience of Learning and Teaching (SELT) surveys as well as GOS surveys and Program reviews.
SELTs are an important source of information to inform individual teaching practice, decisions about teaching duties, and course and program curriculum design. They enable the University to assess how effectively its learning environments and teaching practices facilitate student engagement and learning outcomes. Under the current SELT Policy (http://www.adelaide.edu.au/policies/101/) course SELTs are mandated and must be conducted at the conclusion of each term/semester/trimester for every course offering. Feedback on issues raised through course SELT surveys is made available to enrolled students through various resources (e.g. MyUni). In addition aggregated course SELT data is available.
- Academic Support with Maths
- Academic Support with writing and speaking skills
- Student Life Counselling Support - Personal counselling for issues affecting study
- International Student Support
- AUU Student Care - Advocacy, confidential counselling, welfare support and advice
- Students with a Disability - Alternative academic arrangements
- Reasonable Adjustments to Teaching & Assessment for Students with a Disability Policy
Policies & Guidelines
This section contains links to relevant assessment-related policies and guidelines - all university policies.
- Academic Credit Arrangement Policy
- Academic Honesty Policy
- Academic Progress by Coursework Students Policy
- Assessment for Coursework Programs
- Copyright Compliance Policy
- Coursework Academic Programs Policy
- Elder Conservatorium of Music Noise Management Plan
- Intellectual Property Policy
- IT Acceptable Use and Security Policy
- Modified Arrangements for Coursework Assessment
- Student Experience of Learning and Teaching Policy
- Student Grievance Resolution Process
Students are reminded that in order to maintain the academic integrity of all programs and courses, the university has a zero-tolerance approach to students offering money or significant value goods or services to any staff member who is involved in their teaching or assessment. Students offering lecturers or tutors or professional staff anything more than a small token of appreciation is totally unacceptable, in any circumstances. Staff members are obliged to report all such incidents to their supervisor/manager, who will refer them for action under the university's student’s disciplinary procedures.
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