BIOTECH 7050 - Protein Purification: Principles and Practice
North Terrace Campus - Semester 2 - 2024
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General Course Information
Course Details
Course Code BIOTECH 7050 Course Protein Purification: Principles and Practice Coordinating Unit Molec & Biomedical Science Term Semester 2 Level Postgraduate Coursework Location/s North Terrace Campus Units 3 Contact Up to 7 hours per week Available for Study Abroad and Exchange Y Restrictions Available to students enrolled in the Master of Biotechnology (Biomedical), the Master of Biotechnology (Biomedical) (Advanced), the Master of Biopharmaceutical Engineering and the Graduate Certificate in Biotechnology (Biomedical) only Assessment Assessment of laboratory proficiency, laboratory note-book, practical reports, peer assessment, and tests Course Staff
Course Coordinator: Dr Kate Wegener
Course Timetable
The full timetable of all activities for this course can be accessed from Course Planner.
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Learning Outcomes
Course Learning Outcomes
On successful completion of this course, students will be able to:
1. Demonstrate sound knowledge of current protein purification techniques used in biomedical research and the biotechnology industry
2. Demonstrate practical laboratory skills in chromatography and protein purification.
3. Document laboratory procedures and data effectively in an electronic notebook.
4. Interpret and critically analyse experimental data relating to protein purification.
5. Effectively communicate results and conclusions to a broad audience.
University Graduate Attributes
This course will provide students with an opportunity to develop the Graduate Attribute(s) specified below:
University Graduate Attribute Course Learning Outcome(s) Attribute 1: Deep discipline knowledge and intellectual breadth
Graduates have comprehensive knowledge and understanding of their subject area, the ability to engage with different traditions of thought, and the ability to apply their knowledge in practice including in multi-disciplinary or multi-professional contexts.
1,2,3,4 Attribute 2: Creative and critical thinking, and problem solving
Graduates are effective problems-solvers, able to apply critical, creative and evidence-based thinking to conceive innovative responses to future challenges.
1,2,4,5 Attribute 3: Teamwork and communication skills
Graduates convey ideas and information effectively to a range of audiences for a variety of purposes and contribute in a positive and collaborative manner to achieving common goals.
3,5 Attribute 4: Professionalism and leadership readiness
Graduates engage in professional behaviour and have the potential to be entrepreneurial and take leadership roles in their chosen occupations or careers and communities.
1,2,3,4,5 Attribute 5: Intercultural and ethical competency
Graduates are responsible and effective global citizens whose personal values and practices are consistent with their roles as responsible members of society.
2 Attribute 7: Digital capabilities
Graduates are well prepared for living, learning and working in a digital society.
2, 3, 4, 5 Attribute 8: Self-awareness and emotional intelligence
Graduates are self-aware and reflective; they are flexible and resilient and have the capacity to accept and give constructive feedback; they act with integrity and take responsibility for their actions.
3,4,5 -
Learning Resources
Required Resources
No specific text book is required. A laboratory practical manual specific to the course with detailed experimental methodology will be provided. Suggested reading lists, journal articles, and web sites will be provided as required.
Online Learning
MyUni:
Course resources as provided including video/audio recording of workshops and copies of PowerPoint slides, as well as additional reading/recommended texts
Weblinks:
As specified during the course
Scientific literature and journal articles -
Learning & Teaching Activities
Learning & Teaching Modes
This course will be delivered by the following means:
1 x 3 hour workshop per fortnight
1 x 6 hour practical session per fortnight
Workshops and practical sessions are in alternate weeks.Workload
The information below is provided as a guide to assist students in engaging appropriately with the course requirements.
The information below is provided as a guide to assist students in engaging appropriately with the course requirements.
Contact Hours (48 hours)
Workshops 6 x 3 = 18 hours
Practicals 6 x 6 = 36 hours
Non-contact Hours (108 hours)
Reading/other study hours = 24 hours
Preparation for practicals 2 hour per fortnight = 12 hours
Preparation of lab-notebooks = 12 hours
Reports = 36 hours
Test preparation= 24 hours
Total = approximately 156 hoursLearning Activities Summary
The course involves undertaking practical experience of protein purification using a variety of chromatographic techniques, including affinity, ion exchange, hydrophobic interaction, and size exclusion chromatography. Students will record their activities and results in an e-notebook and analyse and present their results as a report suitable for industry or a research field. Understanding of the techniques will be demonstrated through two written tests, conducted during the course. -
Assessment
The University's policy on Assessment for Coursework Programs is based on the following four principles:
- Assessment must encourage and reinforce learning.
- Assessment must enable robust and fair judgements about student performance.
- Assessment practices must be fair and equitable to students and give them the opportunity to demonstrate what they have learned.
- Assessment must maintain academic standards.
Assessment Summary
Assessment Task Type Due Weighting Learning Outcome Laboratory proficiency Formative and Summative Week 12 10 1,2 E Notebook Formative and Summative Week 12 10 2,3 Tests Formative and Summative Week 6/12 2 x 20 1,4 Practical Reports Formative and Summative Week 7/12 2 x 20 1,4,5 Assessment Detail
Students will carry out a series of wet laboratory based practical sessions to purify a number of proteins. Students will be asked to compare the advantages and disadvantages of the different methods used.
Laboratory Proficiency (total of 10%)
Students will be assessed on their knowledge of key laboratory skills via quizzes following the laboratory sessions (10%). This will ensure students are proficient with the techniques used.
E Notebook (10%)
Students will submit electronic notebook entries at the end of each practical session and will be assessed on their submissions (10% total). Feedback will be given after the first entry. This will allow students to improve their laboratory and record keeping skills.
Practical Reports (40%).
Students will prepare an initial 2000 word report on the purification of one protein at the end of week 6 (15 %). This interim report will give students feedback on their report writing skills that they can utilise in the writing of their final 4000 word report (week 13, 25%).
Tests (40%)
Tests will be used to assess student understanding of the experimental theory and methods that they have learned in lectures and carried out in the practical sessions. These will be given in weeks 6 and 12 (2 x 20% of final grade).
Submission
Late Submission
If an extension is not applied for, or not granted then a penalty for late submission will apply. A penalty of 10% of the value of the assignment for each calendar day that the assignment is late (i.e. weekends count as 2 days), up to a maximum of 50% of the available marks will be applied. This means that an assignment that is 5 days late or more without an approved extension can only receive a maximum of 50% of the marks available for that assignment.Course Grading
Grades for your performance in this course will be awarded in accordance with the following scheme:
M10 (Coursework Mark Scheme) Grade Mark Description FNS Fail No Submission F 1-49 Fail P 50-64 Pass C 65-74 Credit D 75-84 Distinction HD 85-100 High Distinction CN Continuing NFE No Formal Examination RP Result Pending Further details of the grades/results can be obtained from Examinations.
Grade Descriptors are available which provide a general guide to the standard of work that is expected at each grade level. More information at Assessment for Coursework Programs.
Final results for this course will be made available through Access Adelaide.
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Student Feedback
The University places a high priority on approaches to learning and teaching that enhance the student experience. Feedback is sought from students in a variety of ways including on-going engagement with staff, the use of online discussion boards and the use of Student Experience of Learning and Teaching (SELT) surveys as well as GOS surveys and Program reviews.
SELTs are an important source of information to inform individual teaching practice, decisions about teaching duties, and course and program curriculum design. They enable the University to assess how effectively its learning environments and teaching practices facilitate student engagement and learning outcomes. Under the current SELT Policy (http://www.adelaide.edu.au/policies/101/) course SELTs are mandated and must be conducted at the conclusion of each term/semester/trimester for every course offering. Feedback on issues raised through course SELT surveys is made available to enrolled students through various resources (e.g. MyUni). In addition aggregated course SELT data is available.
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Student Support
- Academic Integrity for Students
- Academic Support with Maths
- Academic Support with writing and study skills
- Careers Services
- International Student Support
- Library Services for Students
- LinkedIn Learning
- Student Life Counselling Support - Personal counselling for issues affecting study
- Students with a Disability - Alternative academic arrangements
- YouX Student Care - Advocacy, confidential counselling, welfare support and advice
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Policies & Guidelines
This section contains links to relevant assessment-related policies and guidelines - all university policies.
- Academic Credit Arrangements Policy
- Academic Integrity Policy
- Academic Progress by Coursework Students Policy
- Assessment for Coursework Programs Policy
- Copyright Compliance Policy
- Coursework Academic Programs Policy
- Elder Conservatorium of Music Noise Management Plan
- Intellectual Property Policy
- IT Acceptable Use and Security Policy
- Modified Arrangements for Coursework Assessment Policy
- Reasonable Adjustments to Learning, Teaching & Assessment for Students with a Disability Policy
- Student Experience of Learning and Teaching Policy
- Student Grievance Resolution Process
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Fraud Awareness
Students are reminded that in order to maintain the academic integrity of all programs and courses, the university has a zero-tolerance approach to students offering money or significant value goods or services to any staff member who is involved in their teaching or assessment. Students offering lecturers or tutors or professional staff anything more than a small token of appreciation is totally unacceptable, in any circumstances. Staff members are obliged to report all such incidents to their supervisor/manager, who will refer them for action under the university's student’s disciplinary procedures.
The University of Adelaide is committed to regular reviews of the courses and programs it offers to students. The University of Adelaide therefore reserves the right to discontinue or vary programs and courses without notice. Please read the important information contained in the disclaimer.
