Dr Steven Polyak

Dr Steven Polyak
  • Biography/ Background

    2001 - current Research Fellow, School of Molecular and Biomedical Science, University of Adelaide

    1999 - 2001 Research Associate, Hanson Institute

    2000 PhD University of Adelaide

  • Teaching Interests

    Lecturing Courses

    Biomedical Science IIB

    Masters Biotechnology

  • Research Interests

    Protein structure and function         

    Small molecule drug discovery

    Biotin (vitamin B7 or H)



  • Publications

    Refereed Journal Articles



    Selected papers

    van Marrewijk, L.M., Hijnen, M., Polyak, S.W., Shin, Y., Kamenecka, T.M., Griffin, P.R. & Bruning, J.B. (2016) Structural and Kinetic Mechanism of a Non-Acid PPAR Partial Agonist: SR2067. ACS Chem. Biol. 11(1):273-83

    Azhar, A., Booker G.W. & Polyak, S.W. (2015) Mechanisms of biotin transport. Biochem. Anal Biochem. 4 (4):210

    Salaemae, W., Yap, M.Y., Wegener, K.L., Booker, G.W., Wilce, M.C.J & Polyak, S.W. (2015) Nucleotide triphosphate promiscuity in Mycobacterium tuberculosis Dethiobiotin Synthetase. Tuberculosis. 95(3):259-66

    Tieu, W., Polyak, S.W., Paparella, A.S., Yap, M., Soares da Costa, T., Ng, B., Wang, G., Lumb, R., Bell, J., Turnidge, J.D., Wilce, M.C.J., Booker, G.W. & Abell, A.D. (2015) An improved synthesis of biotinol-5’-AMP: Implications for antibacterial discovery. ACS Med Chem Letts. 6(2):216-220

    Paparella, A.P., Soares da Costa, T.P., Yap, M.Y., Tieu, W., Wilce, M.C.J., Booker, G.W., Abell, A.D. & Polyak, S.W. (2014) Structure guided design of biotin protein ligase inhibitors for antibiotic discovery. Curr. Top. Med. Chem., 14(1): 4-20

    Soares da Costa, T.P., Yap, M.Y., Perugini. M.A., Wallace, J.C., Abell, A.D., Wilce, M.C.J., Polyak, S.W. & Booker, G.W. (2014) Dual roles for F123 in protein homodimerization and inhibitor binding to biotin protein ligase from Staphylococcus aureus. Mol Microbiol. 91(1):110-120.

    Tieu, W., Soares Da Costa, T.P., Yap, M.Y., Keeling, K.L., Wilce, M.C.J., Wallace, J.C., Booker, G.W., Polyak, S. W. & Abell, A.D. (2013) Optimising in situ click chemistry: the screening and identification of biotin protein ligase inhibitors. Chem. Sci. 4, 3533-353.

    Pendini, N.R., Yap, M.Y., Polyak, S. W., Cowieson, N.P., Booker, G.W., Wallace, J.C., Abell, A.D., Wilce, J.A. & Wilce, M.C.J. (2013) Structural characterisation of Staphylocococcus aureus biotin protein ligase: An antibiotic target. Prot. Sci. 22(6): 762-73

    Soares da Costa, T.P. Tieu, W., Yap, M.Y., Zvarec, O., Bell, J., Turnidge, J.D., Wallace. J.C., Booker, G.W., Wilce, M.C.J., Abell, A.D., Polyak, S. W. (2012) Biotin analogues with antibacterial activity are potent inhibitors of biotin protein ligase. ACS Med. Chem. Letts.

    Soares da Costa, T.P., Tieu, W., Yap, M.Y., Pendini, N.R., Polyak, S. W., Sejer Pedersen, D., Morona, R. Turnidge, J.D., Wallace, J.C. Wilce, M.C.J., Booker, G.W. & Abell, A.D. (2012) Selective inhibition of Biotin Protein Ligase from Staphylococcus aureus. J. Biol. Chem. 287(21):17823-32.

    Mayende, L., Swift, R.D., Bailey, L.M., Soares da Costa, T.P., Wallace, J.C., Booker, G.W. & Polyak, S. W. (2012) A novel molecular mechanism to explain biotin-unresponsive Holocarboxylase synthetase. J Mol. Med. 90(1):81-88

    Polyak, S. W., Abell, A.D., Wilce, M.C.J., Zhang, L. & Booker, G.W. (2012) Structure, function and selective inhibition of bacterial acetyl CoA carboxylase. Appl. Micro. Biotech. 

    Salaemae, W., Azhar, A., Booker, G.W. & Polyak, S. W. (2011) Biotin biosynthesis in Mycobacterium tuberculosis: Physiology, biochemistry and molecular intervention. Protein & Cell. 2(9):691-5

    Bailey, L. M., Wallace, J.C. & Polyak, S. W. (2010) Holocarboxylase synthetase: Correlation of protein localisation with biological function Arch Biochem Biophys 496(1):45-52

    Pendini, N.R., Bailey, L.M., Booker, G.W., Wilce, M., Wallace, J.C. & Polyak, S. W. (2008) Microbial relatives of biotin protein ligase aid in understanding holocarboxylase synthetase deficiency BBA-Proteins and proteomics 784(7-8):973-82

    Bailey, L.M., Ivanov,R., Jitrapakdee, S., Wilson, C.J., Wallace, J.C. & Polyak, S. W. (2008) Reduced half-life of holocarboxylase synthetase from patients with severe mutiple carboxylase deficiency Human Mutation Mutation in Brief #1009, 29:E47-57

    Ng, B., Polyak, S. W., Bird, D., Bailey, L.M., Wallace, J.C. & Booker, G.W. (2008) Escherichia coli biotin protein ligase: Characterisation and development of a high-throughput assay Anal. Biochem. 376(1): 131-6

    Bailey, L.M., Ivanov, R., Wallace, J.C. & Polyak, S. W. (2008) Artifactual detection of biotin on histones by streptavidin Anal. Biochem. 373(1):71-7

    Jitrapakdee, S., Surinya, K., Adina-Zada, A., Polyak, S. W., Stojkoski, C., Smyth, R., Booker, G.W., Cleland, C.C., Attwood, P. & Wallace, J.C. (2007) Conserved Glu40 and Glu433
of the biotin carboxylase domain of yeast pyruvate carboxylase I isoenzyme are essential for the association of tetramers Int. J. Biochem. Cell Biol. 39(11):2120-34

    Polyak, S. W., Chapman-Smith, A., Mulhern, T.D., Cronan, J.E. Jnr. & Wallace, J.C. (2001) Mutational analysis of protein substrate presentation in the post-translational attachment of biotin to biotin domains. J. Biol. Chem. 276 (5): 3037-3045

    Polyak, S. W., Chapman-Smith, A., Brautigan, P. & Wallace, J. C. (1999) Biotin protein ligase from Saccharomyces cerevisiae: the N-terminal domain is required for full activity J. Biol. Chem. 274 (46): 32847-32854

    Polyak, S. W., Forsberg, G., Forbes, B.E., McNeil, K.A., Aplin, S. & Wallace J.C (1997) Introduction of spacer peptides N-terminal to a cleavage recognition motif in recombinant fusion proteins can improve site specific cleavage Prot. Eng. 10 (6) : 615 –619


    Invited book chapters

    Polyak, S. W. & Chapman-Smith, A. (2013) Biotin In Encyclopaedia of Biological Chemistry, 2nd edition. Eds Lennarz, W.J. and Lane, M.D. pp 221-225 [ISBN 978-0-12-378631-9] (http://dx.doi.org/10.1016/B978-0-12-378630-2.00005-0).

    Polyak, S. W., Bailey, L.M., Azhar, A. & Booker, G.W. (2012) Biotin (Vitamin H or B7) In Micronutrients: Sources, properties and health benefits. Eds Betancourt, A.I. & Gaitan, H.F. 65-93 [ISBN 978-1-62100-834-7].


    Polyak, S.W., Tieu, W., Soares da Costa, T.P., Jarrad, A., Yap, M.Y., Pendini, N.R., Morona, R., Wallace, J.C. Wilce, M.C.J., Booker, G.W. & Abell, A.D. Novel AntiMicrobial Compounds, PCT/AU2012/001138, filed 21 September 2012. 

    Polyak, S. W., Wallace, J.C., Stojkoski, C and Booker, GW (2005) Inhibitors of biotin protein ligase. PCT WO 2006056007AU

    Pendini, N.R., Polyak, S. W., Wallace, J.C., Booker, G.W. & Wilce, M. (2007) Crystal structure of a bacterial enzyme Prov. Patent Application 2007906228 filed 13 November 2007

  • Professional Associations

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Entry last updated: Friday, 5 Feb 2016